去铁敏对损伤脊髓一氧化氮合酶表达的影响

Protective effect of desferrioxamine on the NOS expression after SCI in rat

目的研究去铁敏(DFO)对急性脊髓损伤大鼠的神经功能保护作用及对3种一氧化氮合酶(NOS)亚型表达的影响。方法利用Allen's打击模型,致伤力为25gcf(10g×2.5cm),选用SD大鼠36只,随机分为3组(n=12):空白组(C组);脊髓损伤组(生理盐水组,S组);去铁敏治疗组(DFO组)。分别于伤后4小时取材,免疫组化方法检测3种NOS亚型的表达情况,并于伤后5周行BBB评分。结果DFO组及S组伤后上述指标比较差异有统计学意义(P<0.05或P<0.01)。结论去铁敏通过阻断铁离子引发的脊髓组织脂质过氧化反应而减轻脊髓组织的炎症反应,减少诱导型NOS(iNOS)的表达;并通过减轻脊髓组织细胞内钙超载,降低固有型NOS(cNOS)的活性,减少了脊髓组织的继发损伤程度。

Objective The aim of this study is to assess the neuroprotective effect of desferrioxamine and the effect of which on the 3 NOS in secondary lesion after SCI in rat. Mthods Contusion model of SCI using the modified Allen＇s method that resulted in rats with incomplete paraplegia was used. Thirty-six rats were divided into three groups （ n = 12 ） ： control group （ C group ）, SCI group （ S group ）, desferrloxamine group （ DFO group ）. Four hours after trauma, activity of iNOS, nNOS and eNOS had been measured using immunohistochemistry methods in the lesion of spinal cord. The functional status was assessed using Basso-Beattie-Bresnehan （BBB） locomotor rating scale in the fifth week （ C group ）. Results There were significant differences in the activity of iNOS, nNOS, the functional status between C group and the other 2 groups, and there were significant differences in eNOS between S group and C group. There were significant differences in the factors above-mentioned between DFO group and S group. Conclusion The neuroprotective effect of DFO is that DFO can reduce the expression of 3 NOS by inhibiting lipid peroxidation, inflammatory reaction and calcium overload. New therapeutic strategies for SL prevention, based on the modulation of 3NOS, will be evaluated.