沙漠干热环境下猪腹部肠管火器伤后肝组织核因子-κB的表达

Expressions of NF-κB in hepatic tissues following intestinal perforation due to abdominal firearm wound in dry-heat environment of desert in pigs

目的探讨沙漠干热环境下猪腹部肠管火器伤后肝脏组织核因子-κB(NF-κB)的表达和肝功能的变化。方法沙漠干热环境组和常温环境组健康长白仔猪各42头随机等分为对照组和伤后1、2、4、8、12小时和24小时组,实验组建立腹部火器伤肠管穿透模型后,用免疫组化图像分析法测定各组肝脏组织内NF-κB的表达,同时测定血清中谷丙转氨酶(ALT)水平。结果伤后各组肝组织NF-κB表达明显高于对照组,沙漠干热环境组于伤后1小时和4小时出现2个高峰;常温环境组于伤后1小时和8小时出现2个高峰。血清ALT水平于伤后显著增高,并于伤后2小时出现第1个高峰,沙漠干热环境组和常温环境组分别于伤后8小时和12小时出现第2个高峰。结论沙漠干热环境下腹部肠管火器伤后肝脏NF-κB的表达增加并伴随肝功能损伤,而且峰值较常温组提前出现,提示腹部肠管火器伤后靶向NF-κB的干预治疗在沙漠干热环境下应及早进行。

Objective To investigate the expression of NF-κB in hepatic tissues and changes of hepatic function following intestinal perforations due to abdominal firearm wound in dry-heat environment of desert in pigs. Methods A total of 84 Chang - Bai piglets were randomly divided into 7 groups in normal temperature environment group and dry-heat desert environment group respectively： control group and post-wounded l h group ,2h group,4h group ,8h group, 12h group,24h group. The model of intestinal perforations due to abdominal firearm wound was established in wounded groups. Expressions of NF-κB were measured with immunohistochemistry and image analysis in all groups. Serum ALT levels were also determined at the same time. Results Expressions of hepatic NF-κB in wounded groups were significantly elevated compared with control group,there were two peaks in lh group and 4h group in dry-heat desert environment group,and two peaks in 1h group and 8h group in normal temperature environment group. Levels of serum ALT in wounded groups were significantly elevated as compared with control group,the dry-heat desert environment group appeared two ALT peaks at 2h group and 8h group,the normal temperature group appeared two ALT peaks at 2h group and 12h group. Conclusion The expressions of NF-κB in hepatic tissues in pigs of intestinal perforations due to abdominal firearm wound in dry-heat desert environment group were significantly elevated and accompanied with the damage of hepatic function, the peaks of NF-κB and ALT appeared earlier in dryheat desert environment groups than in normal temperature environment group ,which suggested that protective measure of target NF-κB for liver injury should be adopted in abdominal firearm wound in dry-heat desert environment as early as possible.