早、中、晚孕期胎盘因子体外抗HIV-1的研究

Study on the 1st,2nd,and 3rd trimester placental factors against HIV-1 in vitro

目的探讨早、中、晚孕期胎盘因子(PF)体外抗人免疫缺陷病毒-1(HIV-1)及在HIV-1垂直传播中的作用。方法荧光染料Calcien-AM标记的H9/HIV-1ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后,与MT2细胞混合培养,荧光显微镜下观察合胞体的形成;用HIV-1ⅢB感染MT2细胞,并分别与早、中、晚孕期不同稀释浓度的PF作用后,用MTT法检测HIV-1感染细胞的存活率,用ELISA方法检测细胞培养上清中p24抗原水平。结果各孕期PF并不能抑制MT2和H9/HIV-1ⅢB细胞的融合,但可以增加HIV-1感染细胞的存活率及减少HIV-1p24抗原的表达,且效应以早孕期PF最大,中孕期PF其次,晚孕期PF最小,并与剂量呈正相关。结论PF在体外具有抗HIV-1的作用,并呈现孕期和剂量相关性,可能在阻断HIV-1垂直传播中具有一定作用。

Objective To investigate the effects of the 1st, 2nd, and 3rd trimester placental factors （PFs） on human immunodeficiency virus type 1 （ HIV- 1） infection in vitro, and to evaluate their possible roles in HIV- 1 intrauterine infection. Methods Calcein-AM-stained H9/HIV-1ⅢB cells were treated with different concentrations of the 1st, 2nd, and 3rd trimester PFs, respectively, and then mixed with MT2 cells. HIV-1-mediated syncytium formation was observed under fluorescent microscope. MT2 cells were treated with cell-free HIV-1 Ⅲ B, then washed and cultured with different concentrations of the 1st, 2nd, and 3rd trimester PFs respectively. The protecting effect of PFs on HIV- 1-infected cells was determined with MTT; viral replication was evaluated by measuring the levels of p24 antigen in culture supernatants with ELISA. Results PFs did not suppress HIV-1-mediated syncyfium formation, but significantly increased HIV-1-infected cells＇ viability and decreased p24 levels in trimester- and concentration-dependent manner. Condusion PFs can reduce HIV-1 infection in vitro, and may have an important role in reducing HIV-1 vertical transmission via placenta.