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干扰素α抑制乙肝病毒X蛋白诱导的肝癌转移的分子机制研究 被引量：3

Interferon-α inhibits the invasion and metastasis of hepatoceilular carcinoma induced by hepatitis B virus X protein
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摘要 目的研究干扰素α抑制乙肝病毒X蛋白诱导肝细胞癌侵袭转移的分子机制，为肝癌的临床治疗研究提供实验依据。方法采用脂质体法构建pcDNA3．1-HBx质粒转染CCL13细胞，将CCL13／HBx细胞与干扰素-α共培养，并用RT—PCR、Western Blot从mRNA和蛋白质水平检测CCL13／pcDNA3．1、CCL13／HBx、CCL13／HBx—INF—α中p202、PTEN基因表达，Transwell实验检测CCL13／pcDNA3．1、CCL13／HBx、CCL13／HBx—INF—α细胞侵袭能力。结果CCL13／HBx细胞中p202、PTEN基因的mRNA及蛋白表达水平明显低于CCL13／pcDNA3．1细胞和CCL13／HBx—INF—α细胞（P〈0．05），而CCL13／pcDNA3．1细胞与CCL13／HBx—INF—α细胞间表达差异无统计学意义（P〉0．05）。CCL13／HBx细胞通过Matrigel的细胞数[（37．2±3．8）个／HP]明显多于CCL13／pcDNA3．1[（6．4±1．2）个／HP，t=8．369，P〈0．05]和CCL13／HBx—INF—α细胞[（7．6±1．3）个／HP，t=7．256，P〈0．051，而CCL13／pcDNA3．1与CCL13／HBx—INF—α细胞间差异无统计学意义（P〉0．05）。结论干扰素α可能通过上调乙肝病毒X蛋白所介导的p202基因、PTEN基因表达，抑制肝癌细胞的侵袭转移。 Objective To investigate the molecular mechanism by which interferon-α inhibits the invasion and metastasis of hepatocellular carcinoma activated by hepatitis B virus X protein, and to found an experimental basis for clinical treatment of hepatocellular carcinoma. Methods pcDNA3.1-HBx plasmid was transfected into CCL13 cells by lipofectamine in vitro, CCL13/HBx was co-cultured with interferon-α. RT-PCR and Western blot was used to detect the expression of P202,PTEN in CCL13/pcDNA3.1 ,CCL13/ HBx and CCL13/HBx- INF-α at mRNA and protein level. Transwell test was used to detect the invasion ability of CCL13/pcDNA3. 1 ,CCL13/HBx,CCL13/HBx-INF-α. Results The expression of p202,PTEN in CCL13/HBx was significantly lower than that in CCL13/ pcDNA3. 1 and CCL13/ HBx-INF-α both at mRNA and protein level, hut there was no significant difference between CCL13/pcDNA3.1 and CCL13/ HBx-INF-α. More CCL13/ HBx cells passed through matrigel （37. 2 ± 3.8/HP） than CCL13/ pcDNA3. 1 （6. 4 ± 1.2/HP, t = 8. 369, P 〈 0. 05 ） and CCL13/HBx-INF-α （7. 6 ± 1.3/HP, t = 7. 256, P 〈 0. 05 ）, but there was no difference between CCL13/ pcDNA3. 1 and CCL13/ HBx-INF-α （P 〉 0.05 ）. Conclusion Interferon-α may inhibit the metastasis of hepatocellular carcinoma by up-regulating the expression of p202 and PTEN induced by Hepatitis B virus X protein.
作者 杨建青 潘光栋 褚光平 刘强 肖亿 袁林 刘振
机构地区 广西壮族自治区柳州市人民医院普外科
出处 《中华普通外科杂志》 CSCD 北大核心 2008年第1期61-64,共4页 Chinese Journal of General Surgery
基金 广西壮族自治区自然科学基金资助项目（桂科青0542068）
关键词 癌 肝细胞 肿瘤转移 HBX 质粒转染 干扰素-Α Carcinoma, hepatoeellular Tumor metastasis HBx Vector transfeetion Interferon-α
分类号 R686 [医药卫生—骨科学]
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参考文献11

  • 1Kim YC, Song KS, Yoon G, el al. Activated ras oncogene collaborates with HBx gene of hepatitis B virus to transform cells by suppressing HBx-mediated apoptosis. Oncogene, 2001 , 20: 16-23.
  • 2陈国凤,王琳,成军,张健,刘妍,刘敏,张玲霞,李莉.基因表达谱芯片技术筛选HBV DNA多聚酶RNase H反式调节基因[J].中西医结合肝病杂志,2005,15(2):81-84. 被引量：2
  • 3Chung TW, Lee YC, Ko JH, el al. Hepatitis B virus X protein modutates the expression of PTEN by inhibiling the function of p53, a transcriptional activator in liver cells. Cancer Res,2003, 63 : 3453-3458.
  • 4Lin SM, Lin C J, Hsu CW,et al. Prospective randomized controlled study of interferon-alpha in preventing hepatocellular carcinoma recurrence after medical ablation therapy for primary tumors.Cancer, 2004.100:376-382.
  • 5Albini A,Iwamoto Y, Kleinman HK, et al. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res, 1987,47 : 3239-3245.
  • 6J.萨姆布鲁克,DW拉塞尔.分子克隆实验指南.3版.北京:科学出版社,2003:26-98.
  • 7Ni YH, Chang MH, Wang KJ, et al. Clinical relevance of hepatitis B virus genotype in childrcn with chronic infection and hepatocellular carcinoma. Gastrenterology, 2004, 127: 1733- 1738.
  • 8Tang H, Oishi N, Kaneko S, et al. Molecular functions and biological roles of hepatitis B virus X protein. Cancer Sci,2006, 10:977 -983.
  • 9Min W, Ghosh S, Lcngycl P. The interferon-inducible p202 protein as a modulator of transcription : inhibition of NF-kappa B, c-Fos, and c-Jun activities. Mol Cell Biol, 1996,16:359-368.
  • 10Pryde F, Khalili S, Robertson K et al. 53BPI exchanges slowly the sites of DNA damage and appears tu require RNA ior its association with chromatin. J Cell Sci, 2005,118:2043-2055

二级参考文献24

  • 1Walton CM, Wu CH, Wu GY. A ribonuclease H-oligo DNA conjugate that specifically cleaves hepatitis B viral messenger RNA. Bioconjug Chem, 2001, 12:770-775.
  • 2Chen WF, Huang MH, Tzang CH, et al. Inhibitory actions of genisrein in human breast cancer (MCF-7) cells. Biochim Biophys Acta,2003, 1638 (2): 187-196.
  • 3Ishihara K, Yasuda K, Hatayarna T. Molecular cloning, expression and localization of human 105 kDa heat shock protein, hsp105.Biochim Biophys Acta, 1999, 1444 (1) : 138-142.
  • 4Vickers C, Hales P, Kaushik V, et al. Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase. J BiolChem, 2002, 277 (17): 11838-14843.
  • 5Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-convetting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9. Circ Res, 2000, 87 (5): E1-9.
  • 6Weber PJ, Eckhard CP, Gonser S, et al. On the role of thymopoietins in cell proliferation. Immunochemical evidence for new members of the human thymopoietin family. Biol Chem, 1999, 380: 653-660.
  • 7Gonser S, Crompton NE, Weber PJ, et al. TP5 triggers signal transduction involving mitogen activated protein kinases in monocytes.J Recept Signal Transduct Res, 1999, 19:155-166.
  • 8Meyer-Olmn D, Brady KW, Blackard JT, et al. Analysis of the TCR beta variable gene repertoire in chimpm~zees, identification of functional homologs to human pseudogenes. J lmmunol, 2003, 170:4161-4169.
  • 9Kao GD. McKenna WG, Guenther MG, et al. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol, 2003, 160:1017-1027.
  • 10lwabuchi K, Basu BP, Kysela B, et al. Potential role for 53BP1 in DNA end-joining repair through direct interaction with DNA. J Biol Chem, 2003, 278:36487-36495.

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中华普通外科杂志
2008年 第1期

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