摘要
目的探讨三磷酸肌醇（IB）和Fas基因表达变化在quercetin治疗裸鼠移植人肝癌中的作用。方法以裸鼠移植人肝癌为研究对象，对照组腹腔注入含0．04％DMSO的RPMI1640培养基0．05ml／g，Quercetin治疗组腹腔注入quercetin 1mg·kg^-1·d^-1，3周后观察肝癌增长情况，并应用同位素试剂盒检测肝癌组织IR含量，RT—PCR分析癌组织Fas mRNA表达，Western blotting分析肝癌组织Fas蛋白表达。结果治疗组肝癌体积和重量均显著低于对照组[体积（15．8±10．1）mm^3 vs（52．3±26．5）mm^3，t=3．853，P=0．003）；重量（44．8±10．4）mg vs（91．3±31．4）mg，t=3．391，P=0．004]，IP3含量显著低于对照组[（13．4±1．4）pmol／mg protein vs（35．3±6．6）pmol／mg protein，t=8．322，P=0．000]，Fas mRNA表达显著高于对照组[RI（灰度与面积之积的相对强度）0．52±0．13 vs 0．36±0．09，t=2．433，P=0．038]，Fas蛋白表达显著高于对照组[RI（灰度与面积之积的相对强度）1．72±0．27vs1．08±0．15，t=5．056，P=0．000]。结论Quercetin能减少IP3生成，上调肝癌组织Fas基因表达，抑制裸鼠移植人肝癌增长。
Objective To evaluate the role of 1, 4, 5 - trisphosphate inositol （ IP3 ） and fas gene expression during the process quercetin inhibits the growth of transplanted human hepatoeellular carcinoma in a nude mouse model. Methods The nude mouse model was treated with quercetin 1 mg · kg^- 1 · d^- 1 （ip） for 3 weeks, tumor volume and weight was measured, IP3, Fas mRNA, Fas protein were assayed by IP3- [^3H] Birtrak assay, RT-PCR, Western blotting, respectively. Results The tumor volume and weight treated with quercetin was lower than control [ （ 15. 8 ± 10. 1 ）mm^3 vs. （52. 3±26. 5） mm^3 ,t =3. 853 ,P = 0. 003, （44. 8 ± 10. 4） mg vs. （91.3±31.4） mg, t =3. 391 ,P =0. 004] ,IP3 content was lower than that of control [（13.4±1.4） pmol/mg protein vs. （35.3±6.6） pmol/mg protein, t =8.322,P =0.000] ,Fas mRNA expression was higher in group treated with quercetin than that of control（ RI =0. 52±0. 13 vs. RI = 0. 36±0. 09, t = 2. 433, P = 0. 038 ）, Fas protein was higher in group treated with quercetin than that of control [RI=1.72±0.27 vs. 1.08±0. 15,t=5.056,P=0.000]. Conclusion Quercetin inhabits the growth of human hepatocellular carcinoma in the nude mouse model probably by a mechanism reducing IP3 production and up-regulating Fas gene expression.
出处
《中华普通外科杂志》
CSCD
北大核心
2008年第2期111-114,共4页
Chinese Journal of General Surgery
基金
广东省医学科学基金立项课题（A2004537）
