HPLC法测定肾安康胶囊中大黄素、大黄酚和丹参素钠的含量

Determination of Emodin, Chrysophanol and Sodium Danshensu in Shen An Kang Capsules by HPLC

目的建立肾安康胶囊中大黄素、大黄酚和丹参素钠的含量测定方法。方法采用HPLC法测定。测定大黄素和大黄酚采用Lichrospher100C18(4.6mm×250mm,5μm)色谱柱,流动相为甲醇-0.1%高氯酸溶液(85∶15),检测波长为254nm,流速为1mL·min-1,柱温为35℃。测定丹参素钠采用KromasilC18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈-1.2%乙酸溶液(8∶92),检测波长为280nm,流速为0.5mL·min-1,柱温为30℃。结果大黄素的线性范围为0.02016～0.10080μg(r=0.9996),平均回收率为97.70%,RSD=2.38%;大黄酚线性范围为0.03456～0.17280μg(r=0.9999),平均回收率为97.69%,RSD=1.92%;丹参素钠的线性范围为0.1448～0.8688μg(r=0.9996),平均回收率为97.52%,RSD=0.77%。结论该方法准确,重现性好,能有效控制肾安康胶囊的质量。

Objective To establish a method for determining the contents of chrysophanol, emodin and sodium Danshensu in Shen An Kang Capsules（SAKC）. Methods Chrysophanol and emodin contents were determined on Lichrospher100 C18 column （4. 6 mm × 250 mm, 5 μm） with the mobile phase of Methanol- 0. 1% Perchloric acid （85 ： 15） at a flow rate of 1 mL · min^- 1, and the detection wavelength was 254 nm. Sodium Danshensu content was determined on Kromasil column C18 （4.6 mm × 250 mm, 5 μm） with the mobile phase of actonenitirile - 1.2 % Glacial acetic acid （8 ： 92）at a flow rate of 0.5 mL · min^- 1, detection wavelength being 280 nm. Results The linearity of emodin was obtained in the range of 0. 020 16 - 0. 100 80 μg（ r = 0. 999 6）, linearity of chrysophanol obtained in the range of 0. 034 56 - 0. 172 80 μg （ r = 0. 999 9）and linearity of sodium Danshensu obtained in the range of 0. 144 8 - 0. 868 8 μg（ r = 0. 999 6）. The average recovery was 97.70 % with RSD 2. 38 % for emodin, 97. 69 % with RSD 1.92 % for chrysophanol and 97.52 % with RSD 0.77 % for sodium Danshensu. Conclusion The method is accurate, reproducible , and is helpful for the quality control of Shen An Kang Capsules.