摘要
目的研究黄芩经自生酶水解后,总黄酮苷元提取的工艺条件。方法采用正交试验,考察乙醇浓度、用量、渗漉速度等因素,采用HPLC法测定提取物中黄芩素、汉黄芩素、千层纸素-A的含量。结果乙醇浓度为影响黄芩总黄酮苷元提取效果的主要因素。确定提取工艺为以10倍量95%乙醇渗漉,渗漉速度为6mL/kg·min-1。结论在考察的工艺条件下,对酶解后黄芩中总黄酮苷元的提取效率高,纯度可达60%以上。
Objective To study the optimization of extraction technology for total flavonoid -aglycone from Scutellaria baicalensis after enzymatic hydrolysis. Methods With three factors of alcohol concentration, dosage and speed of percolation as the indexes, orthogonal experiment was performed. Concentrations of Baicalein, Wogonin and oroxylin - A were analyzed by HPLC. Results The optimal extraction technology is A2B3C2： extracting the medical material by 10 times（V） 95 % alcohol, the speed being 6 mL/kg per min. Conclusion Under optimal extraction technology, extraction rate of total flavonoid - aglycone is very considerable and the concentration is high to 60 %.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第2期143-145,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
