内源性硫化氢在大鼠心力衰竭中的变化及作用

Changes and Role of Endogenous Hydrogen Sulfide in Rat with Heart Failure

目的:观察心肌内源性硫化氢生成的变化及外源性给予硫化氢供体NaHS对心肌损伤的影响,以探讨硫化氢在心力衰竭中的病理生理意义。方法:结扎冠状动脉前降支诱导大鼠心力衰竭,腹腔给予外源性H2S治疗;HE染色检测心肌损伤程度;亚甲基蓝法测定硫化氢浓度;TBA法测定血浆和心肌脂质过氧化产物丙二醛(MDA)含量;自动生化分析仪检测血清天门冬氨酸氨基转换酶(AST)、磷酸肌酸激酶(CPK)及乳酸脱氢酶(LDH)活性。结果:大鼠冠状动脉结扎导致严重心肌损伤;血浆和心肌组织H2S生成显著减少(P<0.001);血浆和心肌MDA含量明显增加(P<0.05)。血浆AST、CPK和LDH活性显著增加(P<0.05)。给予NaHS治疗后,心肌损伤程度明显减轻;血浆和心肌MDA含量明显降低(P<0.05);血浆AST、CPK和LDH活性显著降低(P<0.05)。结论:H2S可通过减少血浆和心肌MDA的生成而对大鼠心肌缺血导致的心力衰竭具有保护作用。

Objective To investigate the changes of endogenous hydrogen sulfide （H-S） in rat with heart failure （HF） and the effects of exogenous administration of H-S donor sodium hydrosulphide （NariS） on myocardial infarction, so as to explore the pathophysiological significance of H-S in HF. Methods： The experimental HF model was induced by left anterior descending coronary occlusion and then was treated with intraperitoneal injection of H2S; the degree of myocardial infarction was determined by observing hematoxylin-eosin stained slices; the H2S level was measured by methylene blue; the contents of malondialdehyde （MDA） in plasma and myocardial homogenates were detected by TBA; aspartate amino transferase （AST）, creatine phosphokinase （CPK） and lactate dehydrogenase （LDH） activities in serum were determined by automatic biochemistrical analysis apparatus. Results： Severe myocardial infarction was induced by coronary occlusion; H2S production in plasma and heart homogenates were significandy declined （P〈0.001）; the content of MDA in plasma and heart homogenates was inereased （P〈0.05）; AST, CPK and LDH activities in plasma were increased （each P〈0.05）. After treatment with NariS, myocardial infarction was obviously rduced; the contents of MDA in plasma and heart homogenates were markedly declined （each P〈0.05）; AST, CPK and LDH activities in plasma were decreased （each P〈 0.05）. Conclusion： The results suggest that H2S has protective effects in HF following myocardial isehemia in rats by decreasing MDA production in plasma and heart.