摘要
生物组织的低温无损保存是低温生物学的一个重要课题.该文采用串联谐振方法产生交变磁场,在低降温速率条件下,研究交变磁场对KMnO4溶液中冰晶形成过程的影响.发现50 kHz交变磁场对该溶液中冰晶的生长具有显著的抑制作用,并形成了含盐冰.本研究成果对容易实现的低冷冻速率下生物组织的低温无损保存具有重要意义,适合实际应用.
Nondestructive cryogenic preservation has been an importaht research topic in cryobiology. Organisms are mostly composed of aqueous solution. Common methods involve the use of an ultra-rapid cooling rate or a very high concentration of cryoprotectants. Unfortunately, adding cryoprotectants introduces impurities for. biomaterials themselves and uhrarapid freezing is very difficult to obtain the same cooling rate for the surface and interior of big samples. The influence of ice crystal formation process in KMnO4 solution by alternated magnetic field was studied under the condition of low cooling rate. Alternated magnetic field was produced based on series resonance. This study shows that 50 kHz alternated formed. The result of low freezing magnetic field can suppress ice crystal growth in the solution, and salty ice rate cryopreservation easy achieved is of great significance, and useful in practical applications.
出处
《应用科学学报》
CAS
CSCD
北大核心
2008年第2期145-149,共5页
Journal of Applied Sciences
基金
国家自然科学基金资助项目(No.50307005)
关键词
低温保存
冰晶
串联谐振
虚拟仪器
cryopreservation, ice crystal, series resonance, virtual instrument