大蒜素体外抗菌活性研究

Researches of Antibacteria Activities of Allicin in Vitro

为评价大蒜素对动物性病原细菌的抗菌活性,以1×105cfu·mL-1的动物源性的致病性大肠杆菌和金黄色葡萄球菌为受试菌,体外测定大蒜素对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)范围。结果表明:大蒜素对大肠杆菌的MIC和MBC范围分别为200～400mg·mL-1和400～1600mg·mL-1;对金黄色葡萄球菌的MIC和MBC范围分别为12.5～25mg·mL-1和25～50mg·mL-1。证实大蒜素在体外对大肠杆菌和金黄色葡萄球菌具有较好的抑菌和杀菌作用。

In order to evaluated the anti-bacteria activities of allicin,the Escherichia coli and Staphylococcus aureus were used in this study.The minimal inhibitory and bactericidal concentration of allicin to two kind of bacteria were tested by using 1×105cfu·mL^-1 of Escherichia coli and Staphylococcus aureus.The results showed that MIC of allicin to E.coli ranged from 200 to 400 mg·mL^-1 and the MBC was 400-1600 mg·mL^-1.While to the Staphy lococcus aureus,MIC ranged from 12.5 to 25 mg·mL^-1,and the MBC was 25-50 mg ·mL^-1.The results illustrated that allicin had a broad spectrum inhibitory and bactericidal activity in vitro,and it could be used in animal diseases prevention and treating.