淋巴管内皮透明质酸受体-1和血管内皮生长因子-C在喉鳞癌组织中的表达

Expression of lymphatic vessel endothelial hyaluronan receptor-1 and vascular endothelial growth factor-C in laryngeal squamous cell carcinomas

目的探讨喉鳞癌组织中淋巴管内皮透明质酸受体-1(lymphatic vessel endothelial hyaluronan receptor-1,LYVE-1)及血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)的表达及其与临床病理因素的关系。方法分别以酶标链霉亲和素-生物素法和Envision二步法检测40例喉鳞癌组织中LYVE-1,VEGF-C和核增殖抗原Ki67的表达,分析LYVE-1标记的淋巴管的密度、位置、增殖情况及VEGF-C表达与喉鳞癌颈淋巴结转移、T分期及肿瘤细胞分化程度等之间的关系。结果(1)颈淋巴结转移组、T3和T4组瘤巢内LYVE-1(+)管腔密度分别高于非转移组、T1和T2组(t=4.539,P=0.000;t=3.896,P=0.000)。(2)瘤巢内LYVE-1(+)管腔存在Ki67细胞核棕黄色着色,而瘤巢周围LYVE-1(+)管腔未见这一现象。(3)VEGF-C在颈淋巴结转移组与无转移组、T1和T2组与T3和T4组之间的表达差异有统计学意义(χ2=4.286,P=0.038;χ2=4.607,P=0.032);VEGF-C(+)组瘤巢内LYVE-1(+)管腔密度高于VEGF-C(-)组,差异有统计学意义(t=2.128,P=0.044)。结论喉鳞癌组织中LYVE-1标记的肿瘤淋巴管生成与肿瘤浸润、淋巴结转移、VEGF-C表达有关,淋巴管生成及VEGF-C表达在喉癌的发展过程中发挥重要的作用。

Objective To investigate the expression of lymphatic vessel endothelial hyaluronan receptor-1 （ LYVE-1 ） and vascular endothelial growth factor-C （VEGF-C） in the laryngeal squamous cell carcinoma and its relationship with the clinicopathological features. Methods labelled streptavidin-biotin method and Envision two-step method was used to detect the expression of LYVE-1, VEGF-C and the proliferation marker Ki67 in the laryngeal squamous cell carcinoma tissues of 40 patients. The density, location and the proliferation of LYVE-1 marked tumor lymph vessels, as well as the relationship between the expression of VEGF-C and the clinicopathological features such as neck nodal metastasis, T stage and squamous cell histological types was analyzed respectively. Results （ 1）The density of the intratumoral LYVE-1 （ ＋ ） vessels was significantly higher in the cases with neck nodal metastasis, T3 and T4 group than that in the cases without neck nodal metastasis, T1 and T2 group respectively （t =4.539, P=0.000; t =3.896, P=0.000）. （2） Ki67（ ＋ ） immunostaining was found in some of the intratumoral LYVE-1（ ＋ ） vessels, but not in the peritumoral LYVE-1 （ ＋ ） vessels. （3） The expression of VEGF-C in the group with neck lymph node metastasis, T3 and T4 group was significantly higher than that in the group without neck lymph node metastasis, T1 and T2 group respectively （χ^2 = 4. 286, P = 0. 038; χ^2 =4.607, P = 0.032）. The density of intratumoral LYVE-1 （ ＋ ） vessels was higher in the VEGF-C （ ＋ ） group than that in the VEGF-C （ - ） group （t = 2. 128, P = 0. 044）. Conclusions LYVE-1 marked tumor lymphangiogenisis in laryngeal squamous cell carcinoma was related to tumor infiltration, metastasis and VEGF-C expression. The tumor lymphangiogenesis and the expression of VEGF-C played an important role in the development of laryngeal squamous cell carcinoma.