丙型肝炎病毒RNA高变区准种复杂性与干扰素疗效关系的初步探讨

Relationship between hepatitis C virus quasi-species complexity and its response to interferon therapy

目的探讨丙型肝炎病毒高变区1(HCV HVR1)准种复杂性与干扰素疗效的关系,为临床选择应用干扰素抗病毒治疗的适应症及预测疗效提供理论依据。方法采用基因芯片法进行HCV基因分型;采用聚合酶链反应-单链构象多态性分析(SSCP)进行HVR1准种复杂性检测。结果治疗前HCV HVR1准种的SSCP条带数(复杂性)≤3者,经干扰素治疗后,HCV RNA阴转率为77.78%,>3者HCV RNA阴转率为21.43%,两者差异具有统计学意义(P<0.01);治疗有效组SSCP条带数为2.44±0.73,无效组为4.50±0.65,两者差异具有统计学意义(P<0.01)结论感染HCV基因型lb型的慢性丙型肝炎患者HVR1准种复杂性程度越高,对干扰素无应答的可能性越大,是预测干扰素疗效的一个重要因素。

Objective To investigate the relationship between hepatitis C viros（HCV）hyper-variable region 1 （HVR1） quasi-species complexity and the response to interferon （IFN） therapy. Methods The types of HCV were determined by the gene chip technique and the HVR1 quasispecies complexity was determined by the polymerase chain reaction-mediated single strand conformarion polymorphism （SSCP） method. Results After IFN treatment, the HCV RNA negative transformation rates in SSCP bands of HCV HVR1 ≤ 3 and 〉 3（detected prior to the IFN treatment） were 77.78% and 21.43 %, and there were significant differences between them（ P 〈 0.01）. The number of SSCP bands was 2.44 ± 0.73 in the effective group while it was 4.50 ± 0.65 in the ineffective group, also there were significant differences between them（ P 〈 0.01）. Conclusion The high complexity of HCV HVR1 quasispecies indicates non-respanse to IFN therapy in patients with HCV 1 b infection, and it is an important predictive factor for the effectiveness of IFN therapy.