摘要
目的探讨褪黑素(Mel)抗癫痫作用的机制。方法采用匹罗卡品(PILO)诱导大鼠癫痫持续状态(SE)模型,用免疫组化技术检测大鼠SE后4个时相点即6h、48h、72h和7d海马γ-氨基丁酸(GABA)和GABA。受体α5(GABAARα5)亚单位表达的动态变化,以及Mel对其变化的影响。结果PILO组大鼠SE后6h,海马内GABA能神经元和GABAARα5亚单位表达均开始减少,尤其以SE后72h~7d改变最为明显,与对照组比较差异有极显著意义(P〈0.01):而Mel组大鼠在SE后72h-7d,海马各区的GABA能神经元和GABAARα5亚单位表达均显著高于PILO组大鼠(P〈0.05)。结论Mel可能通过上调GABA和GABAARα5亚单位的表达来发挥抗癫痫作用。
Objective To probe into the mechanism of anticonvulsant by melatonin (Mel). Methods The rat models of status epilepticus (SE) were induced by pilocarpine (PILO). Changes of expression of γ-Aminobutyric acid (GABA) and GABAA receptor α5 subunit in the hippocampus of SE rats and the effect of Mel on these changes were observed by immunocytochemistry at post-SE 6, 48, 72 h, 7 d. Results The expression of GABA and GABAA receptor ct5 subunit began to decrease in PILO group at post-SE 6 h. The expression of GABA and GABAA receptor α5 subunit was significantly lower in PILO group than in control group at post-SE 72 h-7 d (P〈0.01). But the expression of GABA and GABAA receptor α5 subunit was significantly higher in Mel group than in PILO group at post-SE 72 h-7 d (P〈0.05). Conclusions Anticonvulsive effect of Mel may be due to its ability to increase the expression of GABA and GABAA receptor α5 subunit.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第3期241-245,共5页
Chinese Journal of Neuromedicine