CMV感染监测在同种异基因造血干细胞移植中的应用

Application of Human Cytomegalovirus Infection in Allogeneic Hematopoietic Stem Cell Transplantation

目的探讨不同方法CMV监测在同种异基因造血干细胞移植中应用的可行性。方法应用荧光定量PCR(FQ-PCR)和酶联免疫(ELISA)试剂盒分别检测13名allo-HSCT受者、170份标本的血浆DNA负荷量和血清IgM抗体。同时应用流式细胞术(FCM)检测94份标本白细胞pp65抗原。结果FQ-PCR、FCM、ELISA检出率分别为:35.51%、30.85%、13.08%。FQ-PCR与FCM方法对CMV感染的阳性检出率差别无统计学意义(P>0.05),ELISA的阳性检出率明显低于FQ-PCR和FCM,其差别具有统计学意义(P<0.05)。结论FCM检测pp65抗原和FQ-PCR检测DNA负荷量可用于allo-HSCT后CMV感染的早期诊断。

Objective To explore the application possibility of different detection methods for eytomegalovirus （CMV） in allogeneie hematopoietie stem cell transplantation （allo -HSCT）. Methods The plasma DNA loading and serum level of IgM antibody against CMV in 170 clinical specimens from 13 allo - HSCT patients were detected by real - time FQ - PCR and ELISA respectively. The pp65 antigen in 94 peripheral blood leukocyte samples was measured by FCM. Results The positive rates of FQ -PCR,FCM and ELISA were 35.51% ,30. 85% and 13.08% respectively. There was no statistical significant difference between the positive rate of FQ - PCR and FCM to the CVM infection amount （ P 〉 0. 05 ）. The positive rate of IgM anti - body detected by ELISA was obviously lower than that of DNA quantized by FQ - PCR and pp65 antigen detected by FCM, but the difference between them showed statistical significance （ P 〈 0. 05 ）. Conclusion FQ - PCR and FCM are reliable methods for monitoring recipient reactive CMV infection of allo - HSCT recipients and are worthy to extensively use for guiding antiviral therapy.