摘要
目的通过对不同浓度葡萄糖刺激下人脐静脉内皮细胞条件培养上清中一氧化氮(NO)活力、内皮素(ET)含量、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力的检测,探讨高糖对内皮细胞的影响及与血管收缩因子内皮素、舒张因子一氧化氮、血氧自由基和自由基的清除剂超氧化物歧化酶之间的关系。方法采用硝酸还原酶法测定NO含量,硫代巴比妥酸比色法测定MDA含量,放射免疫学方法测定ET含量,黄嘌呤氧化酶法测定SOD含量。结果在高糖处理的细胞条件培养上清中SOD、NO活性降低,而MDA及ET含量升高(P<0.05)。结论高糖可诱导内皮细胞活性氧产生增多,抗氧化酶活性下降,促进内皮细胞释放ET,抑制NO合成。
Objective To detect the nitric oxide （NO）, endothelin （ ET）, malondialdehyde （MDA）and superoxide dismutase（SOD）in cultured endothelial cells exposure to different glucose concentration,and investigate the effect of high glucose concentration ET,SOD, NO and MDA. Methods The levels of NO,MDA,ET,and SOD in the supernatants were detected by nitrate reductase test, thiobarbituric acid test, radioimmunity method and xanthine oxidase test, respectively. Results When endothelial cells were cultured in high glucose, the activities of SOD and the level of NO were significantly decreased, but the levels of ET-1 and MDA were markedly increased. Conclusion High glucose disrupts the oxidative equilibrium and decreases the vascular elasticity.
出处
《江西医学院学报》
CAS
2008年第1期26-28,32,共4页
Acta Academiae Medicinae Jiangxi
关键词
高糖
人脐静脉内皮细胞
一氧化氦
内皮素
丙二醛
超氧化物岐化酶
high glucose
human umbilical vein endothelial cell
nitric oxide
malondialdehyde
endothelin
super oxide dismutase
