摘要
A HPLC method for the determination of concentrations of Cocarboxylase Tetrahydrate was established. The HPLC conditions consisted of a PHENOMENEX Luna C18 column (250mm ×4.6mm i.d., 5μm) with column temperature at 30℃, 0.05mol/L NaH2PO4 -acetonitrile (50:1, v/v) as a mobile phase with flow rate of 1.0mL/min and detection at 235 nm. The calibrated linear curve of Cocarboxylase Tetrahydrate was within 11.40μg/mL～288.00μg/mL with the correlation coefficient of 0.9999. Cocarboxylase Tetrahydrate was completely separated from impurities. This accurate and reliable HPLC method is applicable for the quality control of Cocarboxylase tetrahydrate.
A HPLC method for the determination of concentrations of Cocarboxylase Tetrabydrate was established. The HPLC conditions consisted of a PHENOMENEX Luna C18 column （250mm ×4. 6mm i. d. , 5μm） with column temperature at 30℃, 0. 05mol/L NaH2PO4 -acetonitrile （50： 1, v/v） as a mobile phase with flow rate of 1.0mL/min and detection at 235 nm. The calibrated linear curve of Cocarboxyiase Tetrabydrate was within 11. 40μg/mL - 288.00μg/mL with the correlation coefficient of 0. 9999. Cocarboxylase Tetrabydrate was completely separated from impurities. This accurate and reliable HPLC method is applicable for the quality control of Cocarboxylase tetrabydrate.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2008年第3期360-362,共3页
Chemical Research and Application
