恶臭假单胞菌对硝基苯污染河水的修复研究

Bioremediation of Nitrobenzene-contaminated River Water Using Pseudomonas Putida

利用被硝基苯污染的某河流底泥中分离出的一株高效降解硝基苯的恶臭假单胞菌,对硝基苯污染河水的修复进行了研究,考察了河水中的微生物、营养液的投加、温度和硝基苯浓度等因素对降解菌生长和硝基苯的降解的影响。结果表明:在未投加细菌的实验,硝基苯浓度的降低小于5%;在5℃对河水灭菌和不灭菌的条件下,投加细菌均将20 mg/L的硝基苯在56 h内降解完全;25℃条件下营养液的投加使20 mg/L的硝基苯降解提前4 h,5℃则提前16 h;在25℃不添加任何营养液的情况下,投加的细菌利用河水中的营养物质以1.5 mg/L.h的平均降解速率将160 mg/L硝基苯完全降解,为污染河流生物修复提供了可能。

Bioremediation of the nitrobenzene polluted river water was studied, using the high effective nitrobenzene degrading pseudomonas putida isolated from sediment of nitrobenzene-contaminated river. The effects of strains presence in the river, addition of nutrients, temperature and nitrobenzene concentration on strain-growth and nitrobenzene degradation were studied. The results indicated that in the control experiment, the nitrobenzene concentration decreased by 5 %. At 5℃, pseudomonas putida could mineralize 20 mg/L nitrobenzene in the river water completely in 56 hours, whether sterilized or not. At 25℃, the time of nitrobenzene-degradation was 4 hours shorter when nutrients are added; and 16 hours shorter when it was at 5℃. At 25℃ ,pseudomonas putida degradated 160 mg/L nitrobenzene completely at a rate of 1.5 mg/L h,utilizing nutrient substance of river without adding of nutrients, and showed the potential for the bioremediation of nitrobenzene-contaminated environment.