枯草芽孢杆菌BSPE2501对草坪炭疽病菌的抑制作用(英文)

Inhibitory Effect of Bacillus subtilis BSPE2501 on the Pathogen of Turfgrass Anthracnose Colletortrichum graminicola

测定了枯草芽孢杆菌BSPE2501菌体和在不同培养时间、培养基、培养基量、转速条件下获得的发酵液对草坪炭疽菌的抑制作用。结果表明:BSPE2501菌体对病原菌菌丝生长和孢子萌发均有明显抑制作用;在NB和BPY中培养获得的发酵液的抑制作用高于在LB和PD中的抑制作用,其1倍发酵液对菌丝生长的抑制率分别达到66.2%和65.5%,明显高于LB(53.5%)、PD(45.8%)的抑制率;其孢子萌发抑制率分别为44.6%、43.1%,高于LB(35.2%)、PD(28.6%)的抑制率;在NB中培养72h、培养基量为100 ml、培养转速为150r/min等条件下获得的发酵液的抑菌效果最好,在此条件下获得的1倍发酵液对菌丝生长和孢子萌发的抑制率分别为85.3%和46.9%、75.7%和43.1%、67.2%和43.4%。

The inhibitory effect of Bacillus subtilis BSPE2501 on Colletortrichum graminicola was determined, and the effect of the fermentation broth cultured for different time, in different medium, medium content, and rotation speed was measured as well. The results showed that BSPE2501 had obvious inhibitory effect on the growth of the pathogen mycelium and spore germination; the inhibitory rate of the fermentation broth cultured in NB and BPY diluted 1 time on the growth of the pathogen mycelium could reach 66.2% （NB） and 65.5% （BPY） respectively, which was significantly higher than that in LB（53.5 % ） and PD（45.8 % ）, while the inhibitory rate of fermentation broth cultured in NB or BPY on the spore germination was 44, 6 % and 43.1% respectively, higher than that in LB （35.2%） and PD（28. 6%）; the inhibitory effect of the fermentation broth cultured in NB for 72h in content of 100ml and at rotation speed of 150rpm had was the best, whose inhibitory rate of the fermentation broth diluted 1 time on the growth of the mycelium and spore germination was 85.3% and 46.9%, 75.7% and 43.1%, 67.2% and 43.4% , respectively.