牛磺酸对微囊化肝细胞生长的影响

Influence of taurine on the growth of microencapsulated hepatocytes

目的:微胶囊中存在一定程度的高渗透压胁迫,可造成细胞生长代谢减慢,为改善微囊化肝细胞的生长,尝试应用抗渗透压胁迫物质牛磺酸,观察其对微囊化肝细胞生长的影响。方法:实验于2006-07/08在中国科学院大连化学物理研究所生物医学材料工程实验室完成。将HepG2细胞悬液(非微囊化细胞)和微囊化HepG2分别接种于牛磺酸浓度为0,0.6,0.8和1.2mmol/L的MEM培养液中,在37℃体积分数为0.05的CO2中培养。MTT法测定非微囊化和微囊化HepG2细胞的生长活性,相差显微镜观察囊内细胞生长状态。结果:①非微囊化HepG2细胞:0.6,0.8和1.2mmol/L的牛磺酸对其生长无影响,吸光度值比较差异无显著意义(P>0.05)。②微囊化HepG2细胞:0.6,0.8mmol/L牛磺酸对微囊化肝细胞的生长亦无影响(P>0.05);但1.2mmol/L牛磺酸可以显著改善微囊化肝细胞的生长,其吸光度值高于0mmol/L牛磺酸组(P<0.05),并促进细胞的聚集。结论:1.2mmol/L牛磺酸可以改善微囊化肝细胞的生长并抵制微囊内的高渗透压胁迫对细胞的生长抑制作用。

AIM： Hyper-osmotic stress may occur in the microcapsules, and reduce the metabolism of cells. This study was designed to investigate the influence of taurine, as an osmolarity substance, on the growth of microencapsulated hepatocytes. METHODS： The experiment was done in the Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics of the Chinese Academy of Sciences between July and August in 2006. Suspended HepG2 cells （control group） and microencapsulated HepG2 cells were inoculated into MEM mediums which contained 0, 0.6, 0.8 and 1.2 mmol/L taurine and cultured at 37 ℃ in an atmosphere with 0.05 volume fraction of CO2. Growth activities of suspended HepG2 cells and microencapsulated HepG2 cells were determined by MTT methods, and the growth of microencapsulated HepG2 cells was observed under phase-contrast microscopy. RESULTS：1.Taurine at 0.6, 0.8 and 1.2 mmol/L had insignificant effects on the growth of non-encapsulated HepG2 cells. There was no significant difference in the absorptance value （P 〉 0.05）.2.Taurine at 0.6 and 0,8 mmol/L did not alter the growth of microencapsulated cells （P 〉 0.05）, but taurine at 1.2 mmol/L increased the growth activities and prompted cell aggregation of microencapsulated HepG2 cells, The absorbtance value was also higher compared to that in 0 mmol/L taurine treatment （P 〈 0.05）. CONCLUSION： Taurine at 1.2 mmol/L can significantly facilitate the growth of microencapsulated HepG2 cells and can prevent the inhibition effects of hyper-osmotic stress on cellular growth.