骨唾液酸蛋白与骨形态发生蛋白体内成骨活性的差异

Osteogenic activities of bone sialoprotein versus bone morphogenetic protein in vivo

目的:研究证明,骨唾液酸蛋白可能与骨形成和骨改建有关。实验拟评价骨唾液酸蛋白在大鼠体内的成骨活性并与骨形态发生蛋白相对比。方法:实验于2006-12/2007-04在解放军广州军区广州总医院动物实验中心完成。①实验分组:8周龄雄性SD大鼠24只,随机数字表法分为骨唾液酸蛋白-β-磷酸三钙复合物组,骨形态发生蛋白-β-磷酸三钙复合物组,β-磷酸三钙组,空白对照组,每组6只。②实验方法:应用重组人骨唾液酸蛋白及骨形态发生蛋白2分别与β-磷酸三钙复合并植入直径8mm的大鼠颅骨骨缺损内。其中骨唾液酸蛋白-β-磷酸三钙复合物组植入骨唾液酸蛋白-β-磷酸三钙复合物,骨形态发生蛋白-β-磷酸三钙复合物组植入骨形态发生蛋白-β-磷酸三钙复合物,β-磷酸三钙只植入β-磷酸三钙,空白对照组未植入任何材料。③实验评估:术后8周取出标本,行大体、X射线、组织学观察及各种植入物的碱性磷酸酶活性。结果:纳入大鼠24只,均进入结果分析,各组植入物未完全吸收,周围均未见明显炎症反应。①骨唾液酸蛋白-β-磷酸三钙复合物和骨形态发生蛋白-β-磷酸三钙复合物在大鼠颅骨骨缺损内形成新骨,与宿主骨相连接,但骨唾液酸蛋白-β-磷酸三钙复合物组的成骨量、新骨密度、骨组织成熟程度均较骨形态发生蛋白低。②颅骨缺损植入物中骨唾液酸蛋白-β-磷酸三钙复合物组和骨形态发生蛋白-β-磷酸三钙复合物组的碱性磷酸酶活性均高于β-磷酸三钙组及空白对照组(P<0.01),而骨形态发生蛋白-β-磷酸三钙复合物组的碱性磷酸酶活性比骨唾液酸蛋白-β-磷酸三钙复合物组高(P<0.05)。结论:骨唾液酸蛋白在骨缺损环境中具有成骨效应,与骨形态发生蛋白对比,骨唾液酸蛋白成骨效应较弱。

AIM： Many studies have demonstrated that bone sialoprotein （BSP） may have correlation with bone formation and rebuilding. This study assessed the osteogenic activity of BSP in vivo, and compare with bone morphogenetic protein （BMP）. METHODS： The experiment was performed in the Animal Experimental Center, Guangzhon General Hospital of Guangzhon Military Area Command of Chinese PLA from December 2006 to April 2007. ①Twenty-four 8-week-old male SD rats were randomly divided into BSP- β -tricalcium phosphate （ β -TCP） group, BMP-β -TCP group, β -TCP alone group and blank control group with 6 animals in each group. ②Recombinate human bone sialoprotein （rhBSP） or recombinate human BMP combined with -TCP were implanted into the calvarial defects （diameter, 8 mm） in rats, respectively. The β -TCP group was only implanted with β -TCP into the same sites, and nothing was implanted into the blank control group. ③The implants were harvested at 8 weeks after implantation. Alkaline phosphatase （ALP） activity of the implants was evaluated by radiological, histological, and bieehemlcal examinations. RESULTS： Twenty-fonr rats were all included in the final analysis. The graft in each group was not absorbed completely, and no apparent inflammatory reaction was found. ①In BSP- β -TCP and BMP- β -TCP groups, new bone formed in the calvarial defects of rats, and the new bone was linking to host bone, but the bone formation mass, density of new bone and maturity degree of bone tissue in BSP-β-TCP were all lower than BMP group. ②The ALP activity in BSP-β-TCP and BMP-β-TCP group was significantly higher than β -TCp group and blank group （P 〈 0.01）, and the ALP activity in BMP- β -TCP group was remarkably higher than BSP- β -TCP group （P 〈 0.05）. CONCLUSION： BSP shows evident osteogenic effect in the model of bone defects, but its effect is inferior compared to the proved bone growth factor-BMP.