地被菊直接体细胞胚发生研究

Direct somatic embryogenesis in ground-cover chrysanthemum

该文建立了地被菊品种‘玉人面’直接体细胞胚受体体系。试验以‘玉人面’叶片为外植体,研究了培养基、生长调节剂等因子对其直接体细胞胚诱导的影响,同时还进行了抗生素敏感性试验。结果表明：①在相同浓度下,KT诱导体胚的效果优于6-BA;②体胚诱导培养基为MS＋KT 2.0 mg/L＋2,4-D 2.0 mg/L＋NAA 0.5 mg/L,诱导10 d后,转入去除2,4-D的体胚发育培养基MS＋KT 2.0 mg/L＋NAA 0.5 mg/L中培养30 d,体胚发生率最高为93.0%,平均每外植体分化的体胚数为13.6个;③MS基本培养基对‘玉人面’体胚发生的效果优于N6、B5培养基;④‘玉人面’直接体胚发生途径的基因转化的卡那霉素选择压为10 mg/L,头孢霉素前期（3-4周内）选择压为300 mg/L,后期（4周后）为100 mg/L。

In this paper, acceptor system of direct somatic embryogenesis in ground-cover chrysanthemum cv. Yurenmian was established. The authors studied the effects of media, hormones on direct somatic leaf segments as explants, and experiments of the sensitivity of antibiotics were also processed. Results showed that： 1 ） KT was more efficient than 2, 4-D in induction somatic embryogenesis at the same concentration ; 2） somatic embryogenesis induction medium： MS ＋ KT 2. 0 mg/L ＋ 2, 4-D 2.0 mg/ L ＋ NAA 0.5 mg/L. After induction 10 days, explants were transferred into somatic embryogenesis development medium, which was MS plus KT 2.0 mg/L and NAA 0.5 mg/L for 30 days. The highest rate of somatic embryogenesis reached 93.0 %, average number of somatic embryogenesis per leaf segment explant was 13.6; 3） MS basic medium was more efficient than N6 and B5 media for direct somatic embryogenesis; 4） experiments of the sensitivity of antibiotics for gene transformation of direct somatic embryogenesis in groundcover chrysanthemum cv. Yurenmian showed： the selection concentration of kanamycin was 10 mg/L, the selection concentration of cefotaxime was 300 mg/L in former period （during 3-4 weeks） and 100 mg/L later （after four weeks）.