结核性腹膜炎和肝癌患者的腹水与血清趋化因子测定及其意义

Clinical significance on chemotactic factor detection in peritoneal effusion and serum of tuberculous peritonitis and liver cancer patients

目的研究结核性腹膜炎,肝癌所引起的腹水和腹水中趋化因子—调节活化正常T细胞表达和分泌因子(RANTES)和基质细胞衍生因子(SDF-1),以及它们的受体CCR3和CXCR4的表达水平及临床意义。方法用酶联免疫吸附法(ELISA)和流式细胞仪(FCM)测定16例结核性腹膜炎,39例肝癌患者以及正常对照20例血清和/或腹水中RANTES、SDF-1和受体CCR3、CXCR4的水平。结果结核性腹膜炎血清及腹水中SDF-1以及CXCR4升高,而肝癌患者血清及腹水中RANTES以及受体CCR3水平上升。结论趋化因子在腹部结核和肿瘤时会出现不同性质T细胞免疫应答,为以后的应用奠定实验基础。

Objective The expression of regulated on activation normal T cell expressed and secreted（RANTES）, stromal cell - derived factor- 1 （SDF - 1 ） and their receptors CCR3 and CXCR4 in patients with tuberculous peritonitis and hepatecellular carcinoma were analyzed, so as to find out the role of chemeokines and their receptors in the pathophysiologieal process of tuberculous and malignant effusion. Methods The concentration of RANTES, SDF- 1 in serum and ascites samples from 16 tuberculous peritonitis and 39 hepatecellular carcinoma patients were determined by enzyme - labeled immunerbent assay（ELISA） and were compare with healthy donors, their receptor CCR3, CXCR4 were analysed by flow cytometer. Results Levels of SDF - 1 and CXCR4 protein in both serum and ascites in patients with tuberculous peritonitis were significantly increased. However, the expression of RANTES and CCR3 were markedly higher in hepatecellular carcinoma patients than those in tuberculous ascites. Conclusions chemeokines and receptors play a different T cell＇s immune response role in tuberculous and malignant ascites.