慢性束缚应激对昆明小鼠MFC移植瘤血管形成的影响

Effect of chronic restraint stress on angiogenesis of MFC xenografts in Kunming mice.

目的探讨慢性束缚应激对昆明小鼠移植瘤血管形成的影响。方法将60只昆明小鼠随机分为正常对照组、单纯肿瘤组、单纯束缚组及肿瘤加束缚组,每组15只,建立相应的慢性束缚应激模型和MFC移植瘤模型,种瘤后10d处死小鼠,测量移植瘤体重量,酶联免疫吸附试验(ELISA)检测小鼠血清VEGF水平,免疫组织化学法检测移植瘤组织VEGF蛋白表达和微血管密度(MVD)。结果与单纯肿瘤组相比,肿瘤加束缚组的移植瘤生长较快,肿瘤增长率达51.08%(P<0.01);慢性束缚应激可促进小鼠血清VEGF水平升高和移植瘤组织中VEGF与MVD的表达。结论慢性束缚应激可通过血管形成来促进MFC移植瘤的生长。

Objective To investigate the effect of chronic restraint stress on angiogenesis of MFC xenografts in Kunming mice. Methods All Kunming mice were randomized into 4 groups（each n = 15） ：normal control,chrenic restraint stress group,tumor group, tumor plus chronic restraint stress group. Chronic restraint stress model .and MFC xenografts model were established in correlated groups. Mice were killed ten days after inoculation of tumor cells. The weight of MFC xenografts were measured, the concentrations of VEGF in serum were detected by enzyme-linked immunoabsorbent assay（ELISA） ,the expression of VEGF and MVD in MFC xenografts were detected by immunohistochemistry. Results Compared with tumor group, MFC xenografts growth faster in tumor plus chronic restraint stress group, the tumor growth rate was 51.08 % （P 〈 0.01 ）. Chronic restraint stress can elevate the serum level of VEGF and induce the expression of VEGF and MVD in MFC xenografts. Conclusion Chronic restraint stress can impact the angiogenesis to promote the growth of MFC xenografts in Kunming mice.