外周血淋巴细胞CR1分子定量测定及其临床应用

Quantitative assay of peripheral blood lymphocytes CR1 and its clinical applications.

目的建立一种定量测定外周血淋巴细胞补体受体1型(PBLCR1)分子的敏感性、特异性、重复性良好的实验方法,并探讨其临床意义。方法常规法分离PBL,并定量包被于V型板中,依次加入抗CR1单克隆抗体、碱性磷酸酶(AP)标记二抗及可溶性底物显色,移显色液于比色孔,405nm比色,计算吸光度值(A)。结果正常人PBLCR1分子表达数量A值平均值为(1.26±0.25),显著高于肝细胞癌(0.72±0.20)、肝硬化(0.69±0.19)、肾病综合征(0.58±0.17)及系统性红斑狼疮(0.47±0.15)患者(P<0.01);SLE患者PBLCR1分子表达的数量显著低于其他疾病组(P<0.01),差异有统计学意义。结论本方法具有良好的敏感性、特异性和重复性;CR1分子的数量表达可分为高、中、低3种形式;正常人为高表达;HCC和LC患者以中、低表达为主;肾病综合征和SLE患者表达显著降低。PBLCR1分子定量测定,对免疫相关性疾病的辅助诊断与治疗效果的评价具有重要的临床意义。

Objective To establish a sensitive,specific and reproductive quantitative measurement of peripheral blood lymphocytes complement receptor type 1 （PBLCR1）elements, and explore its clinical significance. Methods Separate PBL and quantitative coat in the plate of V shape, adding anfi-CR1 monoclonal antibody, alkaline phosphatase（ AP）-labeled antibody and soluble chromogenic substrate in proper order. Read color change in the hole at 405nm wavelength and calculate absorhance value （ A ） at last. Results The average A value of normal person PBLCR1 expression was（ 1.26 ± 0.25） ,which was higher than hepatocellular carcinoma（0.72 ±0.20） ,liver cirrhnsis（0.69 ±0.19） ,nephrotic syndrome（0.58 ±0.17）and systemic lupus erythemetosus（0.47 ± 0.15 ） patients significantly（ P 〈 0.01 ）. Conclusion This method has characters of better sensitivity, specificity and reproducibility. CR1 expression can be divided into high,medium and low forms. We normal person have high expression levels. Cancer patients have medium or low expression levels. Nephrotic syndrome and SLE patients＇ expression levels are even lower. Quantitative assays of PBLCR1 have important clinical significance to the diagnosis and treatment of immune-related diseases.