2006年河北省病毒性腹泻的病原学监测分析

Aetiology Surveillance of Virus Diarrhea in Hebei Province in 2006

目的研究2006年河北省婴幼儿病毒性腹泻的病原学特点。方法2006年1—12月在卢龙县妇幼保健院、卢龙县中医院和河北省儿童医院共采集0～59个月的腹泻患儿的粪便标本454份,进行轮状病毒、杯状病毒、肠道腺病毒和星状病毒的检测、分析。结果454份标本中,轮状病毒阳性216例,阳性率为47.6%。轮状病毒G血清型检测83份标本,其中以G3型为主,占63.9%(53/83);P基因型检测62份标本,其中以P8型为主,占83.9%(52/62);G/P优势组合型以G3P[8]为主,占62.9%(39/62)。检测轮状病毒阴性标本238份,其中杯状病毒阳性54份,阳性率为22.7%;肠道腺病毒阳性32份,阳性率为13.4%;星状病毒阳性8份,阳性率为3.4%。结论轮状病毒是2006年河北省婴幼儿病毒性腹泻的主要病原体,主要血清型为G3型;杯状病毒、星状病毒和肠道腺病毒也是重要的病原体。

Objective To study the aetiology characteristics of virus diarrhea among infants by surveillance in Hebei province. Methods We collected 454 diachorema specimens of diarrhea patients ranging in age from 0 to 59 months in Lulong county maternal and child health hospital, Lulong county Chinese medical hospital and Hebei province children＇s hospital. Rotavirus （RV）, human caliciviruses （HuCV） , astrovirus （AstV） and adenovirus （AdeV） were determined by ELISA and PCR. Results Among 454 specimens, 216 were positive （47.6%） ; 83 specimens of rotavirus G tested by serotyping, G3 was the predominan type （53/83; 63.9% ） ; Among 62 specimens of genotype P, P8 was the predominan type （52/62; 83.9% ） ; G3P [ 8] was the predominan type in G/P dominant combination type （39/62; 62. 9% ）. 54 of the 238 negative specimens of RV were with HuCV positive results （22. 7% ）, 8 with AstV positive results （3.4%）, and 32 with AdeV positive results （ 13.4% ）. Conclusion RV is the dominance etiological agent of infantile diarrhea in Hebei province; The predominan serotype is G3; HuCV, AstV and AdeV are also the important etiological agent.