河南新乡地区汉族人群13个短串联重复序列基因座遗传多态性

Genetic polymorphism of 13 short tandem repeats loci of Han people in Xinxiang,Henan province

目的调查河南新乡汉族群体D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D21S11、CSF1PO、FGA、TH01、TPOX、vWA共13个基因座的遗传多态性分布。方法利用聚合酶链式反应和聚丙烯酰胺凝胶电泳技术及银染技术检测STR基因座各等位基因及其基因型。结果13个STR基因座的基因型频率分布符合Hardy—Weinberg平衡。13个STR基因座的个体识别力、杂合度、多态性信息含量、非父排除率分别为0．7991～0．9708、0．6160～0．8862、0．5868～0．8811、0．4753～0．8905，累计个体识别力为0．999999999，累计非父排除率为0．9999998。结论13个STR基因座在群体遗传学研究和法医学个人识别中具有较高的应用价值，可应用于法庭科学中的个体识别和亲权鉴定。

Objective To study the genetic polymorphism of D3S1358,DSS818,D7S820,D8Sl179,D13S317,D16S539, D18S51, D21 S11, CSF1PO, FGA, TH01, TPOX and vWA loci of Han people in Henan province. Methods The allele and genetype of 13 STR loci were analyzed by polymerase chain reaction amplification, polyacrylamide gel electrophoresis and siliver staining. Results The genotype frequency of these 13 STR loci was in Hardy-weinberg equilibrium. The discrimination power（DP） was from 0. 799 1 to 0. 970 8, Heterozygosity was from 0. 616 0 to 0. 886 2 ,polymorphism information content was from 0. 586 8 to 0. 881 1, probability of paternity exclusion （EPP） was from 0. 475 3 to 0. 890 5, and cumulative DP of the 13 STR was 0. 999 999 999,and cumulative EPP was 0. 999 999 8. Conclusion These 13 STR loci can be studied in population genetics, and applied in forensic medicine for individual identification and paternity testing.