金线莲种子培养的研究

Seeds Culture of Anoectochilus roxburghii

金线莲种子在培养基1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L上萌发后形成原球茎,原球茎可以直接发育成幼苗,也可以由原球茎产生愈伤组织,再由愈伤组织发育成类原球茎而分化成幼苗。通过类原球茎可以实现大量增殖,在MS+6-BA1.0 mg/L+NAA0.1 mg/L上培养60 d的增殖倍数达到6.7倍。在培养基MS+IBA0.3mg/L上,金线莲的生根率可达到96.0%。

Seeds of Anoectochilus roxburghii would germinate and develop to protocorms on the medium of 1/2MS＋6-BA1.0 mg/L ＋ NAA1.0 mg/L. The protocorms may develop to seedlings immediately or induce callous and then induce protocorm-liked-bodies which would develop to seedlings also. Anoec-tochilus roxburghii be rapidly propagated by the later method and 6.7 folds of propagation would get on the medium of MS＋6-BA1.0 mg/L＋ NAA0.1 mg/L after subcultured 60 days. Seedling＇s rooting rate was 96.0% on the medium of MS＋IBA0.3 mg/L.