刺五加苷B的大鼠在体肠吸收特性研究

Absorptive property of eleutheroside B in rat intestine

目的:考察刺五加苷B在大鼠肠道各区段的吸收动力学特性、吸收部位及吸收机制,以期为其现代制剂设计提供理论依据。方法:采用大鼠在体肠段灌流实验,研究了刺五加苷B的吸收部位和吸收动力学特征。结果:不同肠段药物的吸收量和校正的肠壁渗透率(Pw*)按十二指肠、空肠、回肠和结肠的顺序依次下降;刺五加苷B经含肠道酶肠灌流液降解后,其含量按十二指肠、空肠、回肠和结肠的顺序依次下降;采用HPLC法,在血浆和胆汁中未检出刺五加苷B。结论:刺五加苷B生物利用度低是因为肠道酶代谢,引起刺五加苷B被降解,而不仅仅是吸收因素。

AIM ： To observe the absorption characteristics, absorption sites, and mechanism of eleutheroside B from Radix acathopanacis senticosi in various intestinal segments in rat and provide theoretical evidences for designing its preparation. METHODS： In situ perfusion method in rats. The absorption characteristics and absorption sites of eleutheroside B in intestine were applied. RESULTS： Absorbed dose and intestinal surface permeation rate of various intestinal segments decreased in turns in duodenum, jejunum, ileum, and colon; The concentration of eleutheroside B degraded through intestinal enzyme declined in turns in same manner. CONCLUSION： The poor bioavailability of eleutheroside B is not only due to absorption but also due to enzymolysis.