摘要
目的:确定注射用冠心宁(丹参、川芎等)的纯化工艺条件。方法:以丹酚酸B、阿魏酸和原儿茶醛总和为考核指标,对树脂类型、上样量、径高比、pH值、药液上样浓度等进行梯度筛选。结果:将药液pH调至3.0,过D101大孔吸附树脂柱(上样量,药材重量:柱体积=1∶2),用4倍柱体积洗脱,弃去,再用3倍柱体积70%乙醇洗脱。结论:本工艺参数可行,可作为注射用冠心宁的工艺纯化方法。
AIM： To study the purified factors of Guanxinning for Injection （Radix et Rhizoma salviae mihiorrhizae, Rhizoma Chuanxiong, etc）. METHODS： The total of salvianolic acid B,protocatchuci aldehyde and ferulic acid were used to evaluate for the study of type of macroporous adsorption resin,sample volume,the ratio of diameter to height, pH value and sample consistency etc. RESULTS： pH value Guanxinning extract was adjusted to 3.0,through D101 resin finally reached the ratio of diameter to height 1： 2,4 times of water was applied to elution and then 3 times of 70% ethanol for re-elution and collected. CONCLUSION ： These factors are approprite for purifying Guanxinning for Injection.
出处
《中成药》
CAS
CSCD
北大核心
2008年第3期361-365,共5页
Chinese Traditional Patent Medicine
关键词
大孔吸附树脂
冠心宁
工艺研究
marcroporous adsorption resin
Guanxinning
technical study
