柱前衍生化HPLC分析白花蛇舌草多糖中单糖组成

Analysis of polysaccharide composition of Hedyotis difusa Willd polysaccarides by pre-column derivatization HPLC

目的:采用微波提取法提取不同产地白花蛇舌草水溶性多糖,并对多糖的含量及组成进行研究。方法:以苯酚-硫酸法测定总糖含量,柱前衍生化高效液相色谱法鉴别和测定其多糖的单糖组成。结果:不同产地药材中多糖含量以广东最高,白花蛇舌草多糖是由鼠李糖、葡萄糖、半乳糖、阿拉伯糖及甘露糖组成的杂多糖。结论:柱前衍生化高效液相色谱法分析白花蛇舌草多糖中单糖组成方法简便,快速,准确。

AIM ： The polysaccharides of Hedyotis difusa Willd of different sources were extracted and its content and composition of the polysaccharide was analyzed. METHODS： The microwave was employed for the aqueous extract of Hedyotis difusa Willd and the content of total sugar was determined by phenol-sulfur test, the monosaccharides composition was determined by HPLC with pre-column derivatization. RESULTS： The polysaccharide obtained from the samples of Guangdong Province had the highest content. The analytic data showed that the composition of monosaccharides in hydrolytic polysaccharides of Hedyotis difusa Willd were mannose, glucose, galactose, arabinose and rhamnose, the average content of polysaccharides was 9.82%. CONCLUSION： The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Hedyotis difusa Willd polysaccharides and the method is simple, quick and accurate.