双波长分光光度法同时测定Cervitec凝胶剂中醋酸氯己定和麝香草酚含量

Simultaneous determination of chlorhexidine acetate and thymol in an antimicrobial varnish by Dual-wavelength Spectrophotomery

目的 探讨双波长分光光度法测定Cervitec涂荆中醋酸氯己定和麝香草酚的含量。结果 测定醋酸氯己定和麝香草酚的双波长分别244nm、289nm和216rim、267nm，线性范围分别是1μg·mL^-1～40μg·mL^-1（r=0．9998）和0．5μg·mL^-1～20μg·mL^-1（r=0．9997）。结论 本法简便，快速，准确，适用于该制剂中醋酸氯己定和麝香草酚的含量测定。

OBJECTIVE To discuss the effect of a dual-wavelength UV-spectrophotometric method for the simultaneous determination of chlorhexidine acetate （CHa） and thymol in an antimicrobial varnish called Cervitee. METHODS With the optimum dual-wavelengths, the parameters of calibration curves for ehlorhexidine acetate （CHa） and thymol was selected at 244nm、289nm and 216nm、267nm, respectively, Beer＇ s law was obeyed over the concentration ranges of 1μg· mL^-1～40μg· mL^-1 with CHa and 0, 5μg· mL^-1～ 20μg· mL^-1 with thymol. CONCLUSION The present method showed good linearity and precision, and was suitable for the simultaneous determination of thymol and CHa in antimierobial varnish.