高效液相色谱法测定血清中氯唑沙宗的浓度

Determination of chlorzoxazone in serum by RP-HPLC

目的 建立测定血清中氯唑沙宗浓度的高效液相色谱法。方法采用迪马C18色谱柱（250×4．6mm，5μm）；流动相：乙腈-O．02MKH2PO4（pH6．3）=9：11（V／V），含0．16％三乙胺；流速：1．0mL·min^-1；紫外检测波长：280nm；柱温：30℃。结果 氯唑沙宗在2、5～12．5μg·mL^-1的浓度范围内线性关系良好，最低检测限为0．4μg·mL^-1，回收率为102．82±4．39％。结论 本法操作简便，灵敏度高。快速可靠，可用于血清中氯唑沙宗的浓度测定。

OBJECTIVE To establish a RP-HPLC method for the determination of chlorzoxazone in serum. METHODS Diamonsil C18 （250 ×4.6mm, 5μm） column was used. The mobile phase consisted of acetonitrile-0.02M buffer phosphate （9 ： 11, pH6.3） and 0.16 % triethylamine, the flow rate was 1.0mL· min^-1. The detection wavelength was 280nm. The column temperature was kept at 30℃. RESULTS The linear range of chlorzoxazone in serum was 2.5～12.5μg· mL^-1. The limit detection was 0.4μg· mL^-1. And the average recovery of the method was 102.82 ± 4.39 %. CONCLUSION The method is simple, rapid, sensitive, and reliable, and it is suitable for the determination of chlorzoxazone in serum.