硫酸氨基葡萄糖对大鼠软骨细胞增殖的影响

Effect of Glucosamin Sulphat on Proliferation of Rat Chondrocyte in Vitro

目的:探讨硫酸氨基葡萄糖(GS)对大鼠软骨细胞体外增殖的影响。方法:采用二次酶消化法获得大鼠关节软骨细胞进行传代培养,并对第四代细胞进行药物干预,利用MTT比色、免疫组化检测增殖细胞核抗原(PCNA)、流式细胞术检测细胞周期等方法检测各代次大鼠软骨细胞和药物干预后第四代软骨细胞的增殖情况。结果:大鼠软骨细胞随传代培养,MTT比色显示增殖呈下降趋势,免疫细胞化学检测PCNA表达减少,流式细胞术显示细胞增殖指数下降;硫酸氨基葡萄糖可以抑制这一趋势,促进传代培养的细胞增殖能力。结论:GS对传代培养的大鼠软骨细胞的增殖能力有明显促进作用。

Objective： The purpose of the present paper was to investigate the effect of glucosamin sulphat（GS） on the proliferation of rat chondrocyte in vitro. Methods ： The rat articular Chondrocytes were isolated by enzyme digestion and sub - cultivated in serial. The 4^th chondrocyte was interfered with GS. The chondrocytes of different generations and the 4^th medicine intefered generation were detected with such methodsas PCNA （ proliferating cell nuclear antigen） , MTT（ methyl thiazolyl tetrazolium） assay for proliferation and now cytometry for analyses of cell cycle distribution and PI（ proliferation index）. Results： With rat chondrocytes sub - cultivated, MTT assay showed that the proliferating ability of chondrocytes decreased, the express of PCNA tapered in the experiment of immunohistochemistry, as well as PI descended in flow cytometry for analyses. GS can inhibit the tendency and strengthened the proliferating ability of Chondrocytes in serial subcultivation. Conclusion： GS can significantly improve proliferation of chondrocytes in serial subcultivation.