蛋白激酶Cη基因A1427C多态性与动脉粥样硬化性脑梗死的关系

Relationship between single nuclestide polymorphisin A1427C in protein kinase Cη gene and atherosclerotic cerebral infarction

目的 探讨江苏省汉族人群蛋白激酶Cη（PKCη）基因A1427C单核苷酸多态性与动脉粥样硬化性脑梗死的关系。方法 选择120例动脉粥样硬化性脑梗死患者,采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法检测PKCη基因A1427C多态性、采用颈动脉彩色多普勒超声检测颈总动脉内膜-中层厚度,并与对照组100例年龄、性别相匹配的非神经科住院患者和健康体检者比较。将所有研究对象的脑梗死危险因素做多元Logistic回归分析。结果脑梗死组中C等位基因频率（40.8%）及CC＋AC基因型频率（65.0%）明显高于对照组（29.5%和49.0%）,P〈0.05;携带C等位基因的个体与非携带C等位基因的个体比较,患脑梗死的OR值为1.649,（95%CI：1.108-2.456）;与AA基因型比较,CC＋AC基因型患脑梗死的OR值为1.933,（95%CI：1.123-3.326）。脑梗死组颈总动脉内膜-中层厚度与对照组比较差异有统计学意义（P〈0.05）。Logistic回归分析显示,吸烟、高脂血症和C等位基因患脑梗死的OR值分别为2.102、2.004及1.857。结论PKCη基因A1427C多态性可能与动脉粥样硬化性脑梗死的发生相关,C等位基因可能是脑梗死发生的独立危险因素。

Objective To explore the relationship between single nucleotide polymorphism （SNP） A1427C in protein kinase Cη （PKCη） gene and atheroselerotie cerebral infarction in Han nationality popu- lation in Jiangsu, China. Methods A total of 120 patients with atheroselerotie cerebral infarction were selected, and SNP A1427C in PKCη gene was detected by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method, and the common carotid intima-media thickness was measured by carotid uhrasonography/Doppler. They were compared with age and sex matched 100 non-neurological inpatients and healthy individuals in a control group. The risk factors of cerebral infarction in all participants were evaluated by multivariate logistic regression analysis. Results The frequencies of C allele （40. 8% ） and CC ＋ AC genotypes （65. 0% ） in the cerebral infarction group were significantly higher than those in the control group （29. 5% and 49.0% ,P 〈 0.05 ） ; The individuals with C allele compared with the individuals without C allele, the odds ratio （OR） of developing cerebral infarction was 1. 649 （95% CI 1. 108 -2. 456）. As compared with the AA genotypes, the OR of developing cerebral infarction in the CC ＋ AC genotypes was 1. 933 （95% CI 1. 123 -3.326）. There was statistical difference between the cerebral infarction group and the control group in common carotid intima-media thickness （ P 〈 0. 05 ）. Logistic regression analysis indicated that smoking, hyperlipidemia and C allele were the independent risk factors for cerebral infarction in Han population in Jiangsu. Conclusion SNP A1427C in PKCη may be associated with the occurrence of atherosclerotic cerebral infarction. The C allele may be a independent risk factor for cerebral infarction.