补阳还五汤对局灶性脑缺血大鼠脑内caveolin1、2的影响

Effects of Buyang Huanwu decoction on caveolin 1 and 2 of cerebral ischemia in rats

目的探讨补阳还五汤对局灶性脑缺血大鼠脑内caveolin1、2的影响。方法采用大脑中动脉阻塞法复制脑缺血模型,将动物随机分为正常组、假手术组、模型组、补阳还五汤组,分别给予不同处理,在12 h、1、3、7 d不同时间点处死动物,采用免疫组织化学法观察caveolin1、2的表达。结果补阳还五汤能显著减轻神经功能缺损;正常脑内有一定量的caveolin1、2表达,缺血后caveolin1、2迅速增加,分别于术后3 d或1 d达高峰,补阳还五汤组显著提高caveolin1、2的表达,在3、7 d时模型组与补阳还五汤组差异有统计学意义(P<0.01)。结论补阳还五汤可调节脑缺血后caveolin1、2的表达,是其促进神经功能恢复的可能机制之一。

Objective To investigate the effects of Buyang Huanwu decoction （BYHW） on expression of caveolinl and 2 of cerebral ischemia in rats. Methods Cerebral ischemia was induced by middle cerebral artery occlusion. The rats were randomly divided into four groups including naive, shame-operated, control and BYHW groups. All the rats were killed in 12 hours and 1, 3 or 7day after operation. The expressions of caveolinl and caveolin2 were measured by immunohistochemical staining. Results BYHW could reduce obviously the injury of nervous functions; expression of caveolinl and 2 positive cells existed in normal brain, caveolin 1 or 2 were enhanced immediately after ischemia and reached to peak at 3 day or 1 day after ischemia respectively; BYHW could increase the number of caveolinl and 2 positive cells at 3 and 7 day after ischemia and treatment （P〈0.01）. Conclusion BYHW is capable of regulating expression of caveolinl and 2 in ischemic brain which may be one of anti-ischemic mechanism of the decoction.