2，2′-联吡啶In(Ⅲ)配合物与DNA作用的电化学研究

Study on the Binding mode of In(bpy)Cl_3·H_2O with DNA by the Methods of Electrochemistry

应用循环伏安法、微分脉冲伏安法和交流阻抗法研究了配合物In(bpy)Cl3.H2O与DNA在Tris-HCl缓冲溶液(pH=7.2)中的相互作用.结果表明:配合物中心In(Ⅲ)离子的循环伏安曲线上呈现一对准可逆的氧化还原波,DNA与配合物作用后,配位中心离子的氧化还原峰电流明显降低,扩散系数减小,电化学反应阻抗增大,式量电位负移,表明该配合物与DNA的作用方式为静电结合.

The complex In（bpy） Cl3·H2O was synthesized. The binding mode of the complex with DNA was studied by the methods of cyclic voltammetry（ CV）, differential pulse voltammetry and ac impedance in the buffer Tris-HCl（ pH = 7.2）. A quasi-reversible redox wave for the central Indium ion from the cyclic vohammograms was shown at a PG electrode. It has been found that after the complex acted with DNA, The complex＇ s formal potential negatively shifted from -0.696 V to -0.717 V, the values of the reduction peak current and apparent diffusion coefficient decreased significantly. The results of differential pulse voltammetry were the same as cyclic vohammetry, and the electrochemical reaction resistance values increased from 143 Ω· cm^2 to 169 Ω· cm^2. The result indicated that the complex In （bpy） Cl3·H2O acted with DNA apparently. The binding mode of the complex with DNA was stable-electricity binding also medium degree exterior bonding mode was manifested.