南美斑潜蝇对不同菜豆品种的偏好性与寄主化学物质含量的关系

Relationship Between Host Plant Preference of Liriomyza huidobrensis for Different Phaseolus vulgaris Varieties and Plant Compound Contents

【目的】明确南美斑潜蝇对不同菜豆品种的偏好性及其与寄主植物化学成分的关系。【方法】采用非自由选择法测定南美斑潜蝇对不同菜豆品种的偏好性;采用丙酮法、蒽酮比色法、考马斯亮蓝法、磷钼酸-磷钨酸比色法及索氏回流法分别测定了叶片叶绿素、可溶性糖、可溶性蛋白、单宁酸及黄酮含量。【结果】南美斑潜蝇对不同菜豆品种的偏好性存在显著差异,聚类分析可以将其分为3类,最喜好的菜豆品种有双丰二号、富田一号架豆、精品架豆、紫花油豆、大诚地豆、日本无筋绿、双丰架豆、世纪豇豆、泰国架豆王、太空青眉、五常大油豆;其次为九寸莲、天马95-33、精选73-8、天马地豆、泰丰架豆王、富田二号架豆、满架莲、泰国架豆95-33、绿龙架豆、秋紫豆、青岛架豆;最次为骏马地豆、华豇八号、天马架龙王、诚牌精龙王、新选九粒白。化学成分分析表明,不同菜豆品种间的叶绿素、可溶性糖、可溶性蛋白、单宁酸及黄酮含量存在显著差异。相关分析表明,南美斑潜蝇对不同菜豆品种的寄主偏好性与叶绿素、可溶性糖及可溶性蛋白含量无相关关系,而与叶片的单宁酸及黄酮含量呈显著负相关关系。【结论】单宁酸和黄酮对南美斑潜蝇的寄主偏好性具有重要影响,其含量越高越不利于南美斑潜蝇的取食或产卵。

【Objective】The present study is to investigate the host plant preference of Liriomyza huidobrensis for different Phaseolus vulgaris varieties and its relationship with the compound contents in P. vulgaris leaves. 【Method】 No-choice method was used to test the host plant preference. Acetone spectrophotometry, anthrone colorimetric method, Coomassie brilliant blue （CBB） G250 staining, phosphor-molybdenum acid-phosphor-tungstenic acid colorimetry and Soxhlet extraction were applied to measure the contents of lamina’s chlorophyll, soluble sugar, soluble protein, tannin acid and flavone, respectively.【Result】The preference of L. huidobrensis for different P. vulgaris varieties was significantly different. The clustering analysis separated the tested P. vulgaris varieties into three groups based on the levels of the preference of L. huidobrensis. The most favourites included P. vulgaris cv. Shuangfengerhao, Futianyihaojiadou, Jingpinjiadou, Zihuayoudou, Dachengdidou, Ribenwujinglv, Shuangfengjiadou, Shijijiangdou, Tai-guojiadouwang, Taikongqingmei and Wuchangdayoudou. The second favourites were P. vulgaris cv. Jiucunlian, Tianma95-33, Jingxuan73-8, Tianmadidou, Taifengjiadouwang, Futian’erhaojiadou, Manjialian, Taiguojiadou 95-33, Lvlongjiadou, Qiuzidou and Qingdaojiadou. The least favourites included P. vulgaris cv. Junmadidou, Huajiangbahao, Tianmajialongwang, Chengpaijinglongwang and Xinxuanjiulibai. Chemical component analysis showed that the contents of chlorophyll, protein, soluble sugar, tannic acid and flavone were significantly different among the tested varieties. Correlation analysis showed that the host preference of L. huidobrersis was significantly and negatively correlated with the contents of tannic acid and flavone, but had no significant correlation with the contents of chlorophyll, soluble protein and soluble sugar in the varieties.【Conclusion】 The tannic acid and flavone in the leaves of kidney bean had an important effect on the host preference of L. huidobrersis. High contents of tannic acid and flavone were not in favor of oviposition or feeding of the adults.