Beclin1在非小细胞肺癌中的表达及临床意义

Expression of Beclin1 in Non-small Cell Lung Cancer and Its Clinical Significance

目的检测人非小细胞肺癌标本中自噬相关基因Beclin1的表达并讨论其意义及与细胞病理类型和临床分期的关系。方法采用免疫荧光染色、RT-PCR和Western blot法检测54例非小细胞肺癌的肺癌组织、癌旁组织、正常肺组织Beclin1蛋白、mRNA表达。结果免疫荧光染色显示Beclin在肺癌组织中表达较低,表达率为8.3%,显著低于癌旁和正常组织(P=0.00)。Beclin1 mRNA在肺癌、癌旁及正常组织中的相对表达量为1.302±0.074、1.691±0.100、1.673±0.081(F=6.6,P=0.002),Western blot检测Beclin1蛋白在肺癌、癌旁、正常组织中的相对表达量分别为3.489±0.293、5.306±0.459、6.329±0.580(F=9.73,P<0.01)。Beclin1蛋白和mRNA表达与肺癌病理组织类型和临床分期无明显关系。结论Beclin1蛋白和mRNA表达在肺癌组织中明显低于癌旁和正常组织,表达量的差异有统计学意义,在癌旁和正常肺组织中表达接近。

Objective To investigate the expression of autophagy related Beclin1 gene in human nonsmall-cell lung cancer tissues. Methods Protein expression of Beclin1 was determined by immunofluorescence staining and Western Blot, mRNA expression were analyzed by RT-PCR. Results Immunofluorescence staining revealed that the levels of Beclin1 expression in lung cancer were significantly lower than those in adjacent noncancerous tissues and normal tissues, the expression rate was 8.3% （P = 0. 000）. The Beclin1 mRNA expression in lung cancer, adjacent noncancerous tissues, normal tissues were 1.302 ± 0. 074, 1. 691 ± 0. 100, 1. 673 ± 0. 081 （F = 6. 6, P = 0. 002）. Beclin1 protein expression in lung cancer, around cancer tissues,normal tissues were 3. 489 ± 0. 293,5.306 ± 0. 459,6. 329 ± 0. 580 （F = 9. 73, P〈0. 01）. There was no dependability among the Beclin1 expression and pathology or clinic stage of lung cancer. Conclusion The protein and mRNA expression of Beclin1 in lung cancer were much lower than in adjacent noncancerous tissues and normal tissues, those differences were statistical significance, but in adjacent noncancerous tissues and normal tissues,the expression were proximal.