HPLC法测定川芎嗪在小鼠血浆、脑、肝中的含量

Determination of Ligustrazine in Rat's Plasma,Brain and Liver by HPLC

目的:建立以高效液相色谱法测定川芎嗪在小鼠血浆、脑、肝中含量的方法。方法:色谱柱为HypersilODS-C18,流动相为甲醇-1·5%冰醋酸溶液(45:55,V/V),流速为1mL·min-1,内标为阿司匹林标准品,紫外检测波长为279nm。结果:在血浆、脑和肝中,川芎嗪检测浓度在0·00625～7·813μg·mL-1范围内与峰面积积分值呈良好线性关系;最低检测限分别为0·5、1·55和1·55ng·mL-1;方法回收率分别为97·26%、96·44%和95·43%,RSD分别为3·40%、4·19%和4·94%。结论:该方法线性、精密度、回收率良好,灵敏度高,简便易行,可用于川芎嗪相关制剂的药动学及相关研究。

OBJECTIVE： To establish an HPLC method for the determination of the contents of Ligustrazine in rat＇ s plasma, brain and liver. METHODS： Ligustrazine was separated on Hypersil ODS- C18 column with aspirin as internal standard. The mobile phase consisted of methanol- 1.5% glacial acetic acid solution （45 ： 55, V/V） at a flow rate of 1.0 mL·min^ -1.The UV detection wavelength was 279 nm. RESULTS： The linear range of ligustrazine in rat＇s plasma, brain and liver was 0.006 25-7.813μg · mL^- 1, The lowest detectable limits were 0.5 ng · mL ^-1,1.55 ng· mL^ -1,and 1.55 ng · mL^- 1 and the average recoveries were 97.26%, 96.44%, and 95.43% respectively with RSD at 3.40%, 4.19% and 4.94%, respectively. CONCLUSION： With good linearity, precision and recovery, the method is sensitive and simple, and suitable for pharmacokinetic study and the research of Ligustrazine preparation.