银杏提取物-羟丙基-β-环糊精包合物的工艺优选及鉴定

Technology Optimization and Identification of Ginkgo Biloba Extracts-hydroxyprop-β-cyclodextrin(GBE-HP-β-CD) Inclusion Compounds

目的:筛选制备银杏提取物与羟丙基-β-环糊精(HP-β-CD)包合物的最佳工艺,并进行包合物的鉴定。方法:采用正交设计试验,以包合率为指标筛选最佳工艺条件,以溶液-搅拌法制备包合物;以相溶解度法、差示扫描量热(DSC)法、红外分光光度法对包合物进行鉴定。结果:最佳包合条件为银杏提取物:HP-β-CD=1·5:1(质量比),搅拌时间为6h,包合温度为50℃。结论:银杏提取物与HP--CD初步被证明形成包合物。HP--CD对药物有较好的增溶作用。

OBJECTIVE： To optimize the preparation technology of Ginkgo biloba extracts - hydroxyprop -β- cyclodextrin（GBE HP-β- CD） inclusion compounds and to identify this inclusion compound. METHODS： The optimum preparation condition was studied by orthogonal test with encapsulation rate as the index of evaluation. The inclusion compounds were prepared with solution- agitating technique. The inclusion compounds were identified by phase solubility diagram, differential scanning calorimetry（DSC） and infrared spectroscopy methods, respectively. RESULTS： The optimum inclusion condition was as follows： the ratio of GBE to HP -β-CD= 1.5 ： 1; agitating time=6 h; inclusion temperature= 50℃. CONCLUSION： It has been preliminarily proved that it is possible for Ginkgo biloba extracts and hydroxyprop -β-cyclodextrin to be made into inclusion compounds. HP-β- CD has satisfactory solublization on Ginkgo biloba extracts.