HPLC法测定降脂宁胶囊中槲皮素的含量

Determination of Quercetin in Jiangzhining Capsules by HPLC

目的:建立以高效液相色谱法测定降脂宁胶囊中槲皮素含量的方法。方法:色谱柱为Shim-packVP-ODSC18(150mm×4·6mm,5μm),流动相为甲醇-0·4%磷酸溶液(52:48),流速为0·8mL·min-1,检测波长为373nm。结果:槲皮素的进样量在0·0189～0·0566μg范围内与峰面积积分值呈良好的线性关系(r=0·9999);平均回收率为98·06%,RSD=1·73%(n=6)。结论:本方法简便、可靠、准确,可用于降脂宁胶囊的质量控制。

OBJECTIVE： To establish an HPLC method for the determination of the content of Quercetin in Jiangzhining capsules. METHODS： The chromatographic column was Shim- pack VP- ODS C18（150 mm × 4.6 mm, 5 μm） .The mobile phase was methanol - 0.4% phosphoric（52 ： 48） at a flow rate of 0.8 mL · min^ -1.The detection wavelength was 373 nm. RESULTS： The linear range of Quercetin was 0.018 9-0.056 6 μg （r = 0.999 9）, with its average recovery rate at 98.06% （RSD = 1.73%, n = 6） .CONCLUSION： The method is simple, reliable and accurate, and suitable for the quality control of Quercetin in Jiangzhining capsules.