摘要
目的:建立以高效液相色谱法测定降脂宁胶囊中槲皮素含量的方法。方法:色谱柱为Shim-packVP-ODSC18(150mm×4·6mm,5μm),流动相为甲醇-0·4%磷酸溶液(52:48),流速为0·8mL·min-1,检测波长为373nm。结果:槲皮素的进样量在0·0189~0·0566μg范围内与峰面积积分值呈良好的线性关系(r=0·9999);平均回收率为98·06%,RSD=1·73%(n=6)。结论:本方法简便、可靠、准确,可用于降脂宁胶囊的质量控制。
OBJECTIVE: To establish an HPLC method for the determination of the content of Quercetin in Jiangzhining capsules. METHODS: The chromatographic column was Shim- pack VP- ODS C18(150 mm × 4.6 mm, 5 μm) .The mobile phase was methanol - 0.4% phosphoric(52 : 48) at a flow rate of 0.8 mL · min^ -1.The detection wavelength was 373 nm. RESULTS: The linear range of Quercetin was 0.018 9-0.056 6 μg (r = 0.999 9), with its average recovery rate at 98.06% (RSD = 1.73%, n = 6) .CONCLUSION: The method is simple, reliable and accurate, and suitable for the quality control of Quercetin in Jiangzhining capsules.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第9期687-688,共2页
China Pharmacy