复方茵黄解毒汤质量标准研究

Quality Standards of Compound Yinhuang Detoxication Decoction

目的:建立复方茵黄解毒汤的质量标准。方法:采用薄层色谱(TLC)法对处方中蒲公英、大黄、黄柏进行定性鉴别;采用高效液相色谱(HPLC)法测定咖啡酸和阿魏酸的含量。结果:TLC中蒲公英、大黄、黄柏斑点清晰;咖啡酸与阿魏酸的检测浓度分别在8～40μg·mL-1(r=0·9997)、8～40μg·mL-1(r=0·9995)范围内与各自峰面积积分值呈良好的线性关系;二者平均回收率分别为99·29%与99·38%,RSD分别为0·81%与0·65%(n=6)。结论:所建标准可用于本品的质量控制。

OBJECTIVE： To establish the quality standards for Compound Yinhuang detoxication decoction. METHODS： Herba Taraxaci, Radix Et Rhizoma Rhei, and Cortex Phellodendri in the formula were identified qualitatively by TLC; the contents of Caffeic acid and FeruLic acid were determined by HPLC. RESULTS： The TLC spots of Herba Taraxaci, Radix Et Rhizoma Rhei, and Cortex Phellodendri were all clear. The linear range of Caffeic acid was 8-40μg· mL^-1（ r = 0.999 7） versus 8-40μg· mL^-1（r = 0.999 5） of Ferulic acid. The average recoveries were 99.29% and 99.38% with RSD at 0.81% and 0.65% （n = 6）, respectively. CONCLUSION： The established standards can be used for the quality control of Compound Yinhuang detoxication decoction.