摘要
目的检测人原发性肝癌ABCG2+亚群细胞并加以分离,对其生物学特性进行初步研究。方法通过PE-ABCG2单克隆抗体标记,流式细胞仪检测、分选ABCG2+细胞,体外培养后进行细胞周期、增殖活性和凋亡的检测。结果原发性肝癌细胞中存在ABCG2+表型,占细胞总数的1.3%;体外培养24 hours,ABCG2+表型有81.48%处于细胞周期的G0/G1期,non-ABCG2-表型为46.05%;ABCG2+表型凋亡率为11.5%,non-ABCG2-为27.9%;体外连续培养7天,ABCG2+表型增殖活性高于non-ABCG2-表型。结论原发性肝癌细胞中存在比例为1.3%的ABCG2+亚群细胞,大多数处于细胞周期的G0/G1期,与non-ABCG2-细胞相比,具有更高的增殖活性和抗凋亡能力。
Objective To detect and isolate ABCG2^＋ cells from primary hepatocelluar carcinoma, and investigate the biological characteristics of fire subtype. Methods ABCG2^＋ cells were detected and isolated using Flow Cytometry. Cell circle, proliferation activity and apoptosis tolerance analysis were performed respectively. Results In the hepatoeellular carcinoma,approximately 1.3% cells were ABCG2^＋ subpopulation. After sorting 24 hours, the proportion of positive group and negative group at G0/C1 stage was 81.48% ,46.05% respectively. The rate of apoptosis of positive group and negative group was 11.5% ,27.9% respectively. Two groups cells were cultured for 7 days, proliferation activity of positive group is stronger than that of negatwe group. Conclusion There is 1.3 % ABCG2^＋ cells in the hepatoeellular carcinoma. The Majority of positive cells were at G0/G1 stage. ABCG2^＋ cells demonstrated high proliferative potential and anti-apoptotie properties compared with those of negative cells.
出处
《中国实验诊断学》
2008年第3期324-327,共4页
Chinese Journal of Laboratory Diagnosis
