噁唑酮诱导实验性结肠炎小鼠模型的建立和评价

Establishment and evaluation of Oxazolone-induced Mice Colitis Model

目的建立噁唑酮诱导的小鼠结肠炎模型并评价其在溃疡性结肠炎(UC)研究中的价值。方法BALB/c小鼠皮肤涂擦0.2 ml 3%噁唑酮致敏2次,5天后用0.15 ml 1%噁唑酮灌肠。监测小鼠疾病活动指数(DAI)、大体形态学评分、组织学损伤评分,并检测病变结肠组织的髓过氧化物酶(MPO)活性、干扰素(IFN)-γ、白细胞介素(IL)-4含量。结果结肠炎模型小鼠的DAI、大体形态学评分、组织学损伤评分和MPO活性均较对照组明显增高,病变组织IL-4含量明显高于对照组,IFN-γ含量显著低于对照组。结论噁唑酮诱导的结肠炎属于2型辅助性T细胞(Th2)型炎症反应,类似于人类UC。噁唑酮诱导的小鼠结肠炎模型可作为UC发病机制研究和筛选有治疗潜力药物的重要工具。

Objective To set up an oxazolone-induced mice colitis model, and to evaluate its value in research study of ulcerative colitis （UC）. Methods BALB/e mice were skin-sensitized with 0.2 ml 3% oxazolone twice, and then 0.15 ml 1% oxazolone was instilled into their colon 5 days later. Monitoring the disease activity index（DAI） ,morphological changes and histological changes of the damaged colonic tissues. The myelopemxidase（ MPO ） activity and the concentrations of interferon（ IFN ）-γ and interleukin（ IL）- 4 were determined. Results In the mice colitis model group, DAI, score of morphological,histological lesions and MPO activity were markedly higher than the controls. The concentration of IL-4 in the damaged colonic tissues was obviously increased, while the concentrations of IFN-γ were decreased. Conclusion Oxazolone-induced colitis is T helper cell type 2（Th2）inflammation, is similar to the human UC. It is concluded that the oxazolone-induced mice colitis model is useful for the study of the pathogenesis of UC and the screening of the efficacious drug.