苯甲酸二聚铜配合物的合成与体外抗肿瘤作用

Synthesis and Antitumor Activity of A New Benzoato Dimeric Cupper(Ⅱ) Complex in vitro

目的合成一种新的二聚铜配合物,探讨其体外抗肿瘤作用。方法由苯甲醛、乙醇、醋酸铜在水溶液中反应合成[Cu2(C7H5O2)4(C2H5O)2];锥虫蓝细胞计数法观察其对K562细胞增殖的抑制作用。结果X-射线单晶衍射结构分析和红外光谱表征证明合成新的二聚铜配合物,该配合物对K562细胞的IC50为17.3μg/mL。结论合成对K562细胞增殖具有一定抑制作用的二聚铜配合物。

Objective To synthesis a new benzoate dimeric cupper（ Ⅱ ） complex and to investigate its antitumor activity in vitro. Methods The title compound [Cu2（C7H5O2）4（C2H5O2] was synthesized by the reaction of benzoic acid, copper acetate and ethanol in aqueous solution. Trypan blue dye exclusion method was used to detect the inhibiting rate of tumor cell growth. Results IR spectrum and single X-ray crystal analysis have revealed that compound has a dimeric structure and Mr = 703.66 g/tool. The complex inhibited the proliferation of K562 cells significantly and dose-dependently in 48 h, IC50 of K562 was 17.3 μg/mL by trypan blue dye exclusion method. Conclusion The new benzoatodimeric cupper（ Ⅱ ） complex synthesized has significantly antitumor activity in vitro.