福州地区儿童急性呼吸道感染与人类冠状病毒NL63

Acute Respiratory Tract Infection Associated with Human Coronavirus-NL63 in Children in Fuzhou

目的探讨福州地区小儿急性呼吸道感染是否与人类冠状病毒NL63(HCoV-NL63)相关。方法收集211份急性呼吸道感染(ARTI)患儿鼻咽分泌物,RT-PCR法对HCoV-NL631b基因进行筛查,阳性者再对HCoV-NL631a基因进行扩增并与GenBank中相关序列进行比较。结果HCoV-NL631b基因阳性标本3份(1.4%),用HCoV-NL631a基因扩增也得到阳性结果,PCR产物测序后与基因库中多株HCoV-NL63Ⅰa基因比较,同源性98%～99%。结论福州地区部分儿童急性呼吸道感染与HCoV-NL63有关。

Objective To investigate whether the novel human coronavirus——NL63 （HCoV- NL63） was related to acute respiratory tract infections（ARTI） in children in Fuzhou area of China. Methods Nasopharyngeal secretion samples were collected from 211 children with ARTI, and screened for HCoV-NL63 by RT-PCR amplified fragments representing the lb and la genes. The positive amplicons were sequenced and analyzed. Results Three（1.4~） out of the 211 samples were positive for 1 b gene of HCoV-NL63, and also showed positive results on la gene. The nucleotide sequencing showed that the PCR products were 98~99~ identilied to that of la gene fragments of HCoV-NL63 from GenBank. Conclusion It is suggested that only some of acute respiratory tract infections in young children in Fuzhou area are related to HCoV-NL63.