瘦素对LPS诱导的小鼠胸腺细胞凋亡的保护作用

Effect of Leptin on the apoptosis of thymocytes induced by LPS

目的:观察瘦素对小鼠胸腺细胞凋亡的保护作用,探讨其作用机制。方法:取出小鼠胸腺细胞,用MTT法检测瘦素对胸腺细胞的毒性作用,采用AnnexinV/PI双染色法流式细胞仪检测细胞凋亡,以RT-PCR检测caspase3 mRNA的表达。结果:瘦素可呈剂量依赖性的减少LPS对胸腺细胞的毒性作用,流式细胞仪检测表明瘦素可抑制LPS诱导的细胞凋亡,其浓度>100ng/ml时有统计学意义。RT-PCR表明加入瘦素后caspase3的表达下降,且呈剂量依赖性。结论:瘦素对LPS诱导的胸腺细胞凋亡有一定的抑制作用。

Objective：To explore the effect of Leptin on the apoptosis of thymocytes induced by lipopolysaccharide（LPS） in mice. Methods： The toxicity of LPS on thyrnocytes was evaluated using MTT colorimetric assay. Thyrnocytes apoptosis was detected using Annexin V- FITC/PI double staining flow cytometry, caspase3 mRNA were measured by RT-PCR.Results： After cuhured for 6 h, apoptosis of thymocytes was identified by flow cytometry. Apoptosis rate in LPS group （55.12 ±0.656） % was significantly higher than that in control group （ 9.58 ± 0.605 ） % （ P 〈 0.05）, and apoptosis rate in Leptin（200 ng/ml） group decreased clearly （ 47.913± 0.492 ） % （ P 〈 0.05 ）. The mRNA expressions of caspase3 were inhibited by Leptin in a dose-dependent mannar. Conclusion： Leptin is able to decrease the apotopsis of thymocytes induced by LPS.