摘要
目的:探讨右归丸对激素诱导的小鼠胸腺细胞凋亡的保护作用及机制。方法:采用Annexin V/PI双染法检测糖皮质激素及右归丸干预后小鼠胸腺细胞凋亡比例变化;同时采用RT-PCR法检测凋亡相关基因Bcl-2、Bax mRNA的表达状态。结果:激素处理小鼠的胸腺细胞凋亡比例较正常对照小鼠明显上升(P<0.01),右归丸干预后小鼠胸腺细胞凋亡率则明显下降(P<0.01);同时,激素处理小鼠胸腺细胞Bcl-2的表达下调,Bcl-2/Bax mRNA比值随之下降;而右归丸干预后Bcl-2 mRNA转录水平明显高于激素处理小鼠(P<0.01),Bax的表达虽无明显改变,Bcl-2/Bax比值较激素处理小鼠明显增高(P<0.05)。结论:右归丸可明显抑制激素诱导的胸腺细胞凋亡,其机制与逆转激素诱导的Bcl-2/Bax表达失衡密切相关。
Objective： To explore the protective mechanisms of You-gui-wan on hydrocortisone-induced apoptosis of murine thymocytes. Methods： Annexin V/PI double stain was applied to analyze the apoptotic states of thymocytes after injection with hydrocortisone coupled with or without administration You-gui-wan. The transcriptions of Bcl-2 and Bax were tested by RT-PCR. Results： The apoptotic rate of thymocytes was seriously increased in hydrocortisone-injected mice contrasted to matched control mice（ P 〈 0.01）. After administration of You-guiwan coupled with HC treatment,the apoptotic thymecytes were significantly decreased compared with hydrocortisone group （ P 〈 0.01）.Meanwhile, the expression of Bcl-2 mRNA was down-regulated by HC and resulted in reduced ratio of Bcl-2/Bax. The expression of Bcl-2 and the ratio of Bcl-2/Bax were up-regulated in You-gui-wan and HC treated mice （ P 〈0.05）. Conclusion：The results suggest that You- gui- wan can significantly protect the thymecytes from hydrocortisone-induced apoptesis, and the mechanism of protection might be partly related to regulation of the imbalance of Bcl-2/Bax.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第3期228-230,235,共4页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(30572351)
山东省中医管理局资助项目(2005-089)
