单核细胞骨调素的表达水平与狼疮肾炎

Expression level of osteopotin and lupus nephritis

目的:研究骨调素(OPN)在狼疮性肾损害中的作用。方法:采用酶联免疫吸附试验(ELISA)检测了90例系统性红斑狼疮(SLE)患者、15例非SLE肾损害患者及30例对照血浆OPN的水平,应用半定量RT-PCR方法,测定以上各组外周血单个核细胞(PBMCs)OPN的表达。结果:狼疮处于疾病活动时PBMCs OPNmRNA表达上调,尤其是狼疮肾炎活动组患者PBMCs OPNmRNA表达增加最为显著(P<0.01),PBMCs OPNmRNA表达不仅与总狼疮疾病活动指数评分呈正相关(r=0.532,P<0.001),而且与狼疮疾病活动指数肾评分呈正相关(r=0.584,P<0.001),而非狼疮肾脏疾病组仅低水平表达,与正常对照组水平相近似(P>0.05)。狼疮活动组血清OPN水平较非活动组及正常对照组均明显升高(P<0.01),但活动性狼疮肾炎组与无肾损伤组之间血清OPN水平的差异无显著性(P>0.05)。结论:狼疮处于疾病活动时PBMCs OPNmRNA表达上调,并与狼疮肾炎的疾病活动情况密切相关。

Objective： To explore the role of osteopotin in lupus nephritis （LN）. Methods： Sere osteopotin（ OPN ）levels were measured by enzyme linked immunosorbent assay in 90 patients with systemic lupus erythematosus （SLE）, 15 non-SLE patients with renal impairment, and 30 healthy volunteers. OPN mRNA expression in peripheral blood mononuclear cells （PBMCs） was also investigated with reverse transcription-polymerase chain reaction semi-quantitative method.Results： The expression of OPN was significantly higher in active LN groups than in all other groups（ P 〈0.01 ） ,and there was a close correlation between OPN expression and the overall SLE disease activity index score （ r = 0.584, P 〈 0.001） and the SLE disease activity index renal score（ r = 0.584, P 〈 0.001） .Low expression of OPN was observed in disease controls and healthy controls. The sera levels of OPN were significantly higher in patients with active disease than in patients with inactive SLE and controls,but no significant difference was found between the active LN groups and non-renal involvement group （ P 〉 0.05）. Conclusion：The expression of PBMCs OPN mRNA is up regulation in active SLE. Meanwhile,its expression levels are correlated with the activity of LN.