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WWOX基因真核细胞表达载体的构建与鉴定 被引量：1

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摘要 目的构建人WWOX基因真核细胞表达载体。方法采用RT-PCR方法,从人新鲜卵巢组织的总RNA中扩增出1245bp的人WWOXcDNA片段,然后用HindⅢ和EcoRI双酶切后定向克隆到真核细胞表达载体pcDNA3.1中,用限制性内切酶酶切分析和DNA序列分析鉴定重组质粒。结果WWOX基因的序列测定结果与文献报道完全一致,其真核细胞表达载体被成功构建。结论成功克隆出WWOX基因,并成功构建其真核细胞表达载体。
作者 闫洪超 陆晓媛 韩秋峪 林新生
机构地区 徐州医学院附属医院妇产科
出处 《江苏医药》 CAS CSCD 北大核心 2008年第3期287-288,共2页 Jiangsu Medical Journal
基金 江苏省高校自然科学研究指导性计划项目资助(06KJD320190)
关键词 WWOX基因 真核细胞表达载体
分类号 R73-3 [医药卫生—肿瘤]
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参考文献7

  • 1Bednarek AK, Laflin KJ, Daniel RL, et al. WWOX, a novel WW Domain-containing protein mapping to human chromosome 16q23. 3-24. 1, a region frequently affected in breast cancer[J]. Cancer Res, 2000,60(8) : 2140-2145.
  • 2Ramos D, Aldaz CM. WWOX, a chromosomal fragile site gene and its role in cancer[J]. Adv Exp Med Biol, 2006,587 (2): 149-159.
  • 3Aqeilan RI, Kuroki T, Pekarsky Y, et al. Loss of WWOX expression in gastric carcinoma[J]. Clin Cancer, 2004,10 (9) : 3053-3058.
  • 4Gourley C, Paige AJ, Taylor KJ, et al. WWOX mRNA expression profile in epithelial ovarian cancer supports the role of WWOX variant 1 as a tumour suppressor, although the role of variant 4 remains unclear[J]. Int J Oncol, 2005,26(6) : 1681- 1689.
  • 5Pluciennik E, Kusinska R, Potemski P, et al. WWOX-the FRA16D cancer gene., expression correlation with breast cancer progression and prognosis[J]. Eur J Surg Oncol, 2006,32 ( 2 ) 153-157.
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同被引文献13

  • 1Bednarek AK, Laflin K J, Daniel RL, et al. WWOX, a novel WW Domain-containing protein mapping to human chromosome16q23. 3-24.1, a region frequently affected in breast cancer[ J]. Cancer Res,2000,60 (5) :2140-2145.
  • 2Kuroki T, Yendamuri S, Trapasso F. The tumor suppressor gene WWOX at FRA16D is involved in pancreatic carcinogenesis [ J ]. Clin Cancer Res, 2004, 10(7) :2459-2464.
  • 3Nunez MI, Ludes-Meyers J, Abba MC, et al. Frequent loss of WWOX expression in breast cancer:correlation with estrogen re- ceptor status[J]. Breast Cancer Res Treat, 2005, 89 (02):99- 105.
  • 4Donati V, Fontanini G, Dell-Omodarme M. WWOX expression in different histologic types and subtypes of non-small cell lung cancer [J]. Clin Can Res, 2007, 13(3) :884-899.
  • 5Chang NS, Schultz L, Hsu LJ, et al. 17β-Estradiol up regulates and activates WOX1/WWOXvl and WOX2/WWOXv2 in vitro : po-tential role in cancerous progression of breast and prostate to a premetastatic state in vivo[ J]. Oncogene, 2005,24(4) :714-723.
  • 6Aqeilan RI, Trapasso F, Hussain S, et al. Targeted deletion of WWOX reveals a tumor suppressor fuction [ J ]. Proc Natl Acad Sci USA, 2007,104(10) :3949-3954.
  • 7Paige A J, Taylor KJ, Taylor C, et al. WWOX:a candidate tumor suppressor gene involved in multiple tumor types [ J ]. Proc Natl Aead Sei USA, 2001,98(20) :11417-11422.
  • 8Gourley C, Paige A J, Taylor K J, et al. WWOXmRNA expresstion profile in epithelial ovarian cancer surports the role of WWOX vari- anti as a tumour suppressor although the role of variant4 remains unclear[ J ]. Int J Oneol, 2005,26 (6) : 1681-1689.
  • 9Brown LA, Irving J, Parker R, et al. Amplification of EMSY, a novel oncogene on 11q13, in high grade ovarian surface epithelial carcinomas [ J ]. Gynecolo Oncol, 2006,100 (2) :264.
  • 10Weissman IL. Stem cells: units of development, units of regenra- tion, and units in evolution [ J ]. Cell, 2000,100 ( 1 ) : 157-168.

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江苏医药
2008年 第3期

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